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The application of CRISPR/Cas technologies to Brassica crops: current progress and future perspectives
Brassica species are a global source of nutrients and edible vegetable oil for humans. However, all commercially important Brassica crops underwent a whole-genome triplication event, hindering the development of functional genomics and breeding programs. Fortunately, clustered regularly interspaced...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Nature Singapore
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9590542/ https://www.ncbi.nlm.nih.gov/pubmed/36304520 http://dx.doi.org/10.1007/s42994-022-00076-3 |
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author | Li, Jun Yu, Xiaoxiao Zhang, Chao Li, Na Zhao, Jianjun |
author_facet | Li, Jun Yu, Xiaoxiao Zhang, Chao Li, Na Zhao, Jianjun |
author_sort | Li, Jun |
collection | PubMed |
description | Brassica species are a global source of nutrients and edible vegetable oil for humans. However, all commercially important Brassica crops underwent a whole-genome triplication event, hindering the development of functional genomics and breeding programs. Fortunately, clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) technologies, by allowing multiplex and precise genome engineering, have become valuable genome-editing tools and opened up new avenues for biotechnology. Here, we review current progress in the use of CRISPR/Cas technologies with an emphasis on the latest breakthroughs in precise genome editing. We also summarize the application of CRISPR/Cas technologies to Brassica crops for trait improvements. Finally, we discuss the challenges and future directions of these technologies for comprehensive application in Brassica crops. Ongoing advancement in CRISPR/Cas technologies, in combination with other achievements, will play a significant role in the genetic improvement and molecular breeding of Brassica crops. |
format | Online Article Text |
id | pubmed-9590542 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Nature Singapore |
record_format | MEDLINE/PubMed |
spelling | pubmed-95905422022-10-26 The application of CRISPR/Cas technologies to Brassica crops: current progress and future perspectives Li, Jun Yu, Xiaoxiao Zhang, Chao Li, Na Zhao, Jianjun aBIOTECH Review Brassica species are a global source of nutrients and edible vegetable oil for humans. However, all commercially important Brassica crops underwent a whole-genome triplication event, hindering the development of functional genomics and breeding programs. Fortunately, clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) technologies, by allowing multiplex and precise genome engineering, have become valuable genome-editing tools and opened up new avenues for biotechnology. Here, we review current progress in the use of CRISPR/Cas technologies with an emphasis on the latest breakthroughs in precise genome editing. We also summarize the application of CRISPR/Cas technologies to Brassica crops for trait improvements. Finally, we discuss the challenges and future directions of these technologies for comprehensive application in Brassica crops. Ongoing advancement in CRISPR/Cas technologies, in combination with other achievements, will play a significant role in the genetic improvement and molecular breeding of Brassica crops. Springer Nature Singapore 2022-07-02 /pmc/articles/PMC9590542/ /pubmed/36304520 http://dx.doi.org/10.1007/s42994-022-00076-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Review Li, Jun Yu, Xiaoxiao Zhang, Chao Li, Na Zhao, Jianjun The application of CRISPR/Cas technologies to Brassica crops: current progress and future perspectives |
title | The application of CRISPR/Cas technologies to Brassica crops: current progress and future perspectives |
title_full | The application of CRISPR/Cas technologies to Brassica crops: current progress and future perspectives |
title_fullStr | The application of CRISPR/Cas technologies to Brassica crops: current progress and future perspectives |
title_full_unstemmed | The application of CRISPR/Cas technologies to Brassica crops: current progress and future perspectives |
title_short | The application of CRISPR/Cas technologies to Brassica crops: current progress and future perspectives |
title_sort | application of crispr/cas technologies to brassica crops: current progress and future perspectives |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9590542/ https://www.ncbi.nlm.nih.gov/pubmed/36304520 http://dx.doi.org/10.1007/s42994-022-00076-3 |
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