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A universal glycoenzyme biosynthesis pipeline that enables efficient cell-free remodeling of glycans
The ability to reconstitute natural glycosylation pathways or prototype entirely new ones from scratch is hampered by the limited availability of functional glycoenzymes, many of which are membrane proteins that fail to express in heterologous hosts. Here, we describe a strategy for topologically co...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592599/ https://www.ncbi.nlm.nih.gov/pubmed/36280670 http://dx.doi.org/10.1038/s41467-022-34029-7 |