Effect of Root Storage and Forcing on the Carbohydrate and Secondary Metabolite Composition of Belgian Endive (Cichorium intybus L. Var. foliosum)

[Image: see text] Belgian endive is grown in a two-step cultivation process that involves growing of the plants in the field, cold storage of the taproots, and a second growth period in dark conditions called forcing to yield the witloof heads. In this study, the changes in the carbohydrate content and the secondary metabolite composition were studied in different tissues of Belgian endive during the cultivation process. Belgian endive heads contain between 336–388 mg/g DW of total soluble carbohydrates, predominantly fructose and glucose. The heads also contain phenolic compounds and terpenoids that give Belgian endive its characteristic bitter taste. The terpenoid and phenolic compound composition of the heads was found to be constant during the cultivation season, regardless of the root storage time. In roots, the main storage carbohydrate, inulin, was degraded during storage and forcing processes; however, more than 70% of total soluble carbohydrates remained unused after forcing. Additionally, high amounts of phenolics and terpenoids were found in the Belgian endive taproots, predominantly chlorogenic acid, isochlorogenic acid A, and sesquiterpene lactones. As shown in this study, Belgian endive taproots, which are currently discarded after forcing, are rich in carbohydrates, terpenes, and phenolic compounds and therefore have the potential for further valorization. This systematic study contributes to the understanding of the carbohydrate and secondary metabolite metabolism during the cultivation process of Belgian endive.