INSERT-seq enables high-resolution mapping of genomically integrated DNA using Nanopore sequencing

Comprehensive characterisation of genome engineering technologies is relevant for their development and safe use in human gene therapy. Short-read based methods can overlook insertion events in repetitive regions. We develop INSERT-seq, a method that combines targeted amplification of integrated DNA...

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Detalles Bibliográficos
Autores principales: Ivančić, Dimitrije, Mir-Pedrol, Júlia, Jaraba-Wallace, Jessica, Rafel, Núria, Sanchez-Mejias, Avencia, Güell, Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9594898/
https://www.ncbi.nlm.nih.gov/pubmed/36284361
http://dx.doi.org/10.1186/s13059-022-02778-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9594898/
https://www.ncbi.nlm.nih.gov/pubmed/36284361
http://dx.doi.org/10.1186/s13059-022-02778-9

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