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INSERT-seq enables high-resolution mapping of genomically integrated DNA using Nanopore sequencing

Comprehensive characterisation of genome engineering technologies is relevant for their development and safe use in human gene therapy. Short-read based methods can overlook insertion events in repetitive regions. We develop INSERT-seq, a method that combines targeted amplification of integrated DNA...

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Detalles Bibliográficos
Autores principales:	Ivančić, Dimitrije, Mir-Pedrol, Júlia, Jaraba-Wallace, Jessica, Rafel, Núria, Sanchez-Mejias, Avencia, Güell, Marc
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2022
Materias:	Method
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9594898/ https://www.ncbi.nlm.nih.gov/pubmed/36284361 http://dx.doi.org/10.1186/s13059-022-02778-9

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