CRISPR-KRISPR: a method to identify on-target and random insertion of donor DNAs and their characterization in knock-in mice

CRISPR tools can generate knockout and knock-in animal models easily, but the models can contain off-target genomic lesions or random insertions of donor DNAs. Simpler methods to identify off-target lesions and random insertions, using tail or earpiece DNA, are unavailable. We develop CRISPR-KRISPR...

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Detalles Bibliográficos
Autores principales: Tanaka, Masayuki, Yokoyama, Keiko, Hayashi, Hideki, Isaki, Sanae, Kitatani, Kanae, Wang, Ting, Kawata, Hisako, Matsuzawa, Hideyuki, Gurumurthy, Channabasavaiah B., Miura, Hiromi, Ohtsuka, Masato
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9594901/
https://www.ncbi.nlm.nih.gov/pubmed/36284311
http://dx.doi.org/10.1186/s13059-022-02779-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9594901/
https://www.ncbi.nlm.nih.gov/pubmed/36284311
http://dx.doi.org/10.1186/s13059-022-02779-8

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