A high-content endogenous GLUT4 trafficking assay reveals new aspects of adipocyte biology

Insulin-induced GLUT4 translocation to the plasma membrane in muscle and adipocytes is crucial for whole-body glucose homeostasis. Currently, GLUT4 trafficking assays rely on overexpression of tagged GLUT4. Here we describe a high-content imaging platform for studying endogenous GLUT4 translocation...

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Detalles Bibliográficos
Autores principales: Diaz-Vegas, Alexis, Norris, Dougall M, Jall-Rogg, Sigrid, Cooke, Kristen C, Conway, Olivia J, Shun-Shion, Amber S, Duan, Xiaowen, Potter, Meg, van Gerwen, Julian, Baird, Harry JM, Humphrey, Sean J, James, David E, Fazakerley, Daniel J, Burchfield, James G
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Life Science Alliance LLC 2022
Materias:
Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9595207/
https://www.ncbi.nlm.nih.gov/pubmed/36283703
http://dx.doi.org/10.26508/lsa.202201585

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9595207/
https://www.ncbi.nlm.nih.gov/pubmed/36283703
http://dx.doi.org/10.26508/lsa.202201585

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