Cargando…

The Combination of Mesyl-Phosphoramidate Inter-Nucleotide Linkages and 2′-O-Methyl in Selected Positions in the Antisense Oligonucleotide Enhances the Performance of RNaseH1 Active PS-ASOs

Antisense oligonucleotides (ASOs) that mediate RNA target degradation by RNase H1 are used as drugs to treat various diseases. Previously we found that introduction of a single 2′-O-methyl (2′-OMe) modification in position 2 of the central deoxynucleotide region of a gapmer phosphorothioate (PS) ASO...

Descripción completa

Detalles Bibliográficos
Autores principales:	Zhang, Lingdi, Liang, Xue-hai, De Hoyos, Cheryl Li, Migawa, Michael, Nichols, Joshua G., Freestone, Graeme, Tian, Jun, Seth, Punit P., Crooke, Stanley T.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Mary Ann Liebert, Inc., publishers 2022
Materias:	Original Papers
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9595634/ https://www.ncbi.nlm.nih.gov/pubmed/35861704 http://dx.doi.org/10.1089/nat.2022.0005

Ejemplares similares

Mesyl phosphoramidate antisense oligonucleotides as an alternative to phosphorothioates with improved biochemical and biological properties
por: Miroshnichenko, S. K., et al.
Publicado: (2019)

Viable RNaseH1 knockout mice show RNaseH1 is essential for R loop processing, mitochondrial and liver function
por: Lima, Walt F., et al.
Publicado: (2016)

Towards next generation antisense oligonucleotides: mesylphosphoramidate modification improves therapeutic index and duration of effect of gapmer antisense oligonucleotides
por: Anderson, Brooke A, et al.
Publicado: (2021)

RNA modifications can affect RNase H1-mediated PS-ASO activity
por: Doxtader Lacy, Katelyn A., et al.
Publicado: (2022)

Translation can affect the antisense activity of RNase H1-dependent oligonucleotides targeting mRNAs
por: Liang, Xue-Hai, et al.
Publicado: (2018)

RNase H1-Dependent Antisense Oligonucleotides Are Robustly Active in Directing RNA Cleavage in Both the Cytoplasm and the Nucleus
por: Liang, Xue-Hai, et al.
Publicado: (2017)

Site-specific incorporation of 5′-methyl DNA enhances the therapeutic profile of gapmer ASOs
por: Vasquez, Guillermo, et al.
Publicado: (2021)

Golgi-58K can re-localize to late endosomes upon cellular uptake of PS-ASOs and facilitates endosomal release of ASOs
por: Liang, Xue-Hai, et al.
Publicado: (2021)

Synthesis, biophysical properties and biological activity of second generation antisense oligonucleotides containing chiral phosphorothioate linkages
por: Wan, W. Brad, et al.
Publicado: (2014)

Understanding the effect of controlling phosphorothioate chirality in the DNA gap on the potency and safety of gapmer antisense oligonucleotides
por: Østergaard, Michael E, et al.
Publicado: (2020)

Site-specific replacement of phosphorothioate with alkyl phosphonate linkages enhances the therapeutic profile of gapmer ASOs by modulating interactions with cellular proteins
por: Migawa, Michael T, et al.
Publicado: (2019)

The rates of the major steps in the molecular mechanism of RNase H1-dependent antisense oligonucleotide induced degradation of RNA
por: Vickers, Timothy A., et al.
Publicado: (2015)

Binding of phosphorothioate oligonucleotides with RNase H1 can cause conformational changes in the protein and alter the interactions of RNase H1 with other proteins
por: Zhang, Lingdi, et al.
Publicado: (2021)

Molecular Mechanisms of Antisense Oligonucleotides
por: Crooke, Stanley T.
Publicado: (2017)

Some ASOs that bind in the coding region of mRNAs and induce RNase H1 cleavage can cause increases in the pre-mRNAs that may blunt total activity
por: Liang, Xue-hai, et al.
Publicado: (2020)

Cleavage of Oligonucleotides Containing a P3’→N5’ Phosphoramidate Linkage Mediated by Single-Stranded Oligonucleotide Templates
por: Ito, Kosuke Ramon, et al.
Publicado: (2011)

Synthesis of Oligonucleotides Carrying Inter-nucleotide N-(Benzoazole)-phosphoramide Moieties
por: Vasilyeva, Svetlana V., et al.
Publicado: (2022)

Antisense Oligonucleotides Capable of Promoting Specific Target mRNA Reduction via Competing RNase H1-Dependent and Independent Mechanisms
por: Vickers, Timothy A., et al.
Publicado: (2014)

RNase H sequence preferences influence antisense oligonucleotide efficiency
por: Kiełpiński, Łukasz J., et al.
Publicado: (2017)

RNase H sequence preferences influence antisense oligonucleotide efficiency
por: Kiełpiński, Łukasz J, et al.
Publicado: (2019)

Defining the Factors That Contribute to On-Target Specificity of Antisense Oligonucleotides
por: Lima, Walt F., et al.
Publicado: (2014)

Stabilin-1 and Stabilin-2 are specific receptors for the cellular internalization of phosphorothioate-modified antisense oligonucleotides (ASOs) in the liver
por: Miller, Colton M., et al.
Publicado: (2016)

NAT10 and DDX21 Proteins Interact with RNase H1 and Affect the Performance of Phosphorothioate Oligonucleotides
por: Zhang, Lingdi, et al.
Publicado: (2022)

Contributions of the two accessory subunits, RNASEH2B and RNASEH2C, to the activity and properties of the human RNase H2 complex
por: Chon, Hyongi, et al.
Publicado: (2009)

Antisense oligonucleotides targeting translation inhibitory elements in 5′ UTRs can selectively increase protein levels
por: Liang, Xue-hai, et al.
Publicado: (2017)

RNaseH-mediated simultaneous piggyback knockdown of multiple genes in adult zebrafish
por: Raman, Revathi, et al.
Publicado: (2020)

Golgi-endosome transport mediated by M6PR facilitates release of antisense oligonucleotides from endosomes
por: Liang, Xue-hai, et al.
Publicado: (2020)

Defining the 5΄ and 3΄ landscape of the Drosophila transcriptome with Exo-seq and RNaseH-seq
por: Afik, Shaked, et al.
Publicado: (2017)

Defining the RNaseH2 enzyme-initiated ribonucleotide excision repair pathway in Archaea
por: Heider, Margaret R., et al.
Publicado: (2017)

RNA cleavage products generated by antisense oligonucleotides and siRNAs are processed by the RNA surveillance machinery
por: Lima, Walt F., et al.
Publicado: (2016)

Kinetic and subcellular analysis of PS-ASO/protein interactions with P54nrb and RNase H1
por: Vickers, Timothy A, et al.
Publicado: (2019)

Solid-Phase Synthesis of Phosphorothioate/Phosphonothioate and Phosphoramidate/Phosphonamidate Oligonucleotides
por: Kostov, Ondřej, et al.
Publicado: (2019)

Evidence that the RNAseH activity of the duck hepatitis B virus is unable to act on exogenous substrates
por: Gong, Yunhao, et al.
Publicado: (2001)

High Flexibility of RNaseH2 Catalytic Activity with Respect to Non-Canonical DNA Structures
por: Dede, Maria, et al.
Publicado: (2021)

Protocol to use RNaseH1-based CRISPR to modulate locus-associated R-loops
por: Khosraviani, Negin, et al.
Publicado: (2022)

Hepatotoxicity of high affinity gapmer antisense oligonucleotides is mediated by RNase H1 dependent promiscuous reduction of very long pre-mRNA transcripts
por: Burel, Sebastien A., et al.
Publicado: (2016)

COPII vesicles can affect the activity of antisense oligonucleotides by facilitating the release of oligonucleotides from endocytic pathways
por: Liang, Xue-hai, et al.
Publicado: (2018)

Identifying and avoiding off-target effects of RNase H-dependent antisense oligonucleotides in mice
por: Hagedorn, Peter H, et al.
Publicado: (2018)

Elucidation of the Biotransformation Pathways of a Galnac(3)-conjugated Antisense Oligonucleotide in Rats and Monkeys
por: Shemesh, Colby S, et al.
Publicado: (2016)

mRNA levels can be reduced by antisense oligonucleotides via no-go decay pathway
por: Liang, Xue-hai, et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_v3uavfsb3tsmldt6om1u2h3fp7