Cargando…

BoostMEC: predicting CRISPR-Cas9 cleavage efficiency through boosting models

BACKGROUND: In the CRISPR-Cas9 system, the efficiency of genetic modifications has been found to vary depending on the single guide RNA (sgRNA) used. A variety of sgRNA properties have been found to be predictive of CRISPR cleavage efficiency, including the position-specific sequence composition of...

Descripción completa

Detalles Bibliográficos
Autores principales:	Zarate, Oscar A., Yang, Yiben, Wang, Xiaozhong, Wang, Ji-Ping
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2022
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9597963/ https://www.ncbi.nlm.nih.gov/pubmed/36289480 http://dx.doi.org/10.1186/s12859-022-04998-z

Ejemplares similares

Boosting plant immunity with CRISPR/Cas
por: Chaparro-Garcia, Angela, et al.
Publicado: (2015)

A cleavage-based surrogate reporter for the evaluation of CRISPR–Cas9 cleavage efficiency
por: Jung, Soo Bin, et al.
Publicado: (2021)

Cholesterol-rich lipid-mediated nanoparticles boost of transfection efficiency, utilized for gene editing by CRISPR-Cas9
por: Hosseini, Elaheh Sadat, et al.
Publicado: (2019)

Sequence features associated with the cleavage efficiency of CRISPR/Cas9 system
por: Liu, Xiaoxi, et al.
Publicado: (2016)

PINCER: improved CRISPR/Cas9 screening by efficient cleavage at conserved residues
por: Veeneman, Brendan, et al.
Publicado: (2020)

Carrier strategies boost the application of CRISPR/Cas system in gene therapy
por: Xu, Zunkai, et al.
Publicado: (2022)

Programmable RNA recognition and cleavage by CRISPR/Cas9
por: O’Connell, Mitchell R., et al.
Publicado: (2014)

CRISPR/Cas9 cleavage of viral DNA efficiently suppresses hepatitis B virus
por: Ramanan, Vyas, et al.
Publicado: (2015)

Conformational control of DNA target cleavage by CRISPR–Cas9
por: Sternberg, Samuel H., et al.
Publicado: (2015)

Nucleosomes inhibit target cleavage by CRISPR-Cas9 in vivo
por: Yarrington, Robert M., et al.
Publicado: (2018)

Increasing on-target cleavage efficiency for CRISPR/Cas9-induced large fragment deletion in Myxococcus xanthus
por: Yang, Ying-jie, et al.
Publicado: (2017)

Engineered circular guide RNAs boost CRISPR/Cas12a- and CRISPR/Cas13d-based DNA and RNA editing
por: Zhang, Xin, et al.
Publicado: (2023)

Structural insights into DNA cleavage activation of CRISPR-Cas9 system
por: Huai, Cong, et al.
Publicado: (2017)

CRISPR/Cas9 editing of APP C-terminus attenuates β-cleavage and promotes α-cleavage
por: Sun, Jichao, et al.
Publicado: (2019)

Efficient cleavage resolves PAM preferences of CRISPR-Cas in human cells
por: Tang, Lianchao, et al.
Publicado: (2019)

A conformational checkpoint between DNA binding and cleavage by CRISPR-Cas9
por: Dagdas, Yavuz S., et al.
Publicado: (2017)

Frequent Aneuploidy in Primary Human T Cells after CRISPR-Cas9 cleavage
por: Nahmad, A.D., et al.
Publicado: (2022)

Element coding based accurate evaluation of CRISPR/Cas9 initial cleavage
por: Hu, Jianyu, et al.
Publicado: (2021)

Machine learning in the estimation of CRISPR-Cas9 cleavage sites for plant system
por: Das, Jutan, et al.
Publicado: (2023)

Editor's cut: DNA cleavage by CRISPR RNA-guided nucleases Cas9 and Cas12a
por: Swartjes, Thomas, et al.
Publicado: (2020)

Flowing through the CRISPR-CAScade: Will genome editing boost cell therapies?
por: Ben-David, Uri
Publicado: (2013)

A machine learning approach for predicting CRISPR-Cas9 cleavage efficiencies and patterns underlying its mechanism of action
por: Abadi, Shiran, et al.
Publicado: (2017)

Prediction of CRISPR/Cas9 single guide RNA cleavage efficiency and specificity by attention-based convolutional neural networks
por: Zhang, Guishan, et al.
Publicado: (2021)

Biased and Unbiased Methods for the Detection of Off-Target Cleavage by CRISPR/Cas9: An Overview
por: Martin, Francisco, et al.
Publicado: (2016)

piCRISPR: Physically informed deep learning models for CRISPR/Cas9 off-target cleavage prediction
por: Störtz, Florian, et al.
Publicado: (2023)

Permanent Inactivation of HBV Genomes by CRISPR/Cas9-Mediated Non-cleavage Base Editing
por: Yang, Yu-Chan, et al.
Publicado: (2020)

Efficient precise knockin with a double cut HDR donor after CRISPR/Cas9-mediated double-stranded DNA cleavage
por: Zhang, Jian-Ping, et al.
Publicado: (2017)

Definition of CRISPR Cas12a Trans-Cleavage Units to Facilitate CRISPR Diagnostics
por: Lv, Hailong, et al.
Publicado: (2021)

Efficient Mitochondrial Genome Editing by CRISPR/Cas9
por: Jo, Areum, et al.
Publicado: (2015)

Comparison of non-canonical PAMs for CRISPR/Cas9-mediated DNA cleavage in human cells
por: Zhang, Yilan, et al.
Publicado: (2014)

A CRISPR/Cas9 Cleavage System for Capturing Fungal Secondary Metabolite Gene Clusters
por: Xu, Xinran, et al.
Publicado: (2021)

Guide RNAs with embedded barcodes boost CRISPR-pooled screens
por: Zhu, Shiyou, et al.
Publicado: (2019)

Highly Efficient Targeted Mutagenesis of Drosophila with the CRISPR/Cas9 System
por: Bassett, Andrew R., et al.
Publicado: (2013)

Highly Efficient Targeted Mutagenesis of Drosophila with the CRISPR/Cas9 System
por: Bassett, Andrew R., et al.
Publicado: (2014)

FrCas9 is a CRISPR/Cas9 system with high editing efficiency and fidelity
por: Cui, Zifeng, et al.
Publicado: (2022)

Programming PAM antennae for efficient CRISPR-Cas9 DNA editing
por: Wang, Fei, et al.
Publicado: (2020)

Simple and Efficient Targeting of Multiple Genes Through CRISPR-Cas9 in Physcomitrella patens
por: Lopez-Obando, Mauricio, et al.
Publicado: (2016)

The Advance of CRISPR-Cas9-Based and NIR/CRISPR-Cas9-Based Imaging System
por: Qiao, Huanhuan, et al.
Publicado: (2021)

Efficient Generation of Myostatin Gene Mutated Rabbit by CRISPR/Cas9
por: Lv, Qingyan, et al.
Publicado: (2016)

CRISPR/Cas9 Mediates Efficient Conditional Mutagenesis in Drosophila
por: Xue, Zhaoyu, et al.
Publicado: (2014)

Cannot write session to /tmp/vufind_sessions/sess_h3td3042pq2kvnsov6tne4aera