Maximizing Cumulative Trypsin Activity with Calcium at Elevated Temperature for Enhanced Bottom-Up Proteome Analysis

SIMPLE SUMMARY: Trypsin is frequently employed to cleave proteins ahead of mass spectrometry characterization. Traditionally, enzyme digestion involves overnight incubation of proteins at 37 °C, which is time consuming though still may yield poor digestion efficiency. While raising the temperature s...

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Detalles Bibliográficos
Autores principales: Nickerson, Jessica L., Doucette, Alan A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9598648/
https://www.ncbi.nlm.nih.gov/pubmed/36290348
http://dx.doi.org/10.3390/biology11101444

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9598648/
https://www.ncbi.nlm.nih.gov/pubmed/36290348
http://dx.doi.org/10.3390/biology11101444

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