Enhancing the Production of Pinene in Escherichia coli by Using a Combination of Shotgun, Product-Tolerance and I-SceI Cleavage Systems

SIMPLE SUMMARY: The limit screening method (LMS method) and product-tolerance engineering was proposed and applied. AcrB, flgFG, motB and ndk were found to be associated with an enhanced tolerance of E. coli to pinene. Using the I-SceI cleavage system, the promoters of acrB, flgFG, and ndk genes wer...

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Detalles Bibliográficos
Autores principales: Huang, Ming-Yue, Wang, Wei-Yang, Liang, Zhen-Zhen, Huang, Yu-Chen, Yi, Yi, Niu, Fu-Xing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9598909/
https://www.ncbi.nlm.nih.gov/pubmed/36290388
http://dx.doi.org/10.3390/biology11101484

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9598909/
https://www.ncbi.nlm.nih.gov/pubmed/36290388
http://dx.doi.org/10.3390/biology11101484

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