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Evidence of Renin–Angiotensin System Receptors in Deep Fascia: A Role in Extracellular Matrix Remodeling and Fibrogenesis?

Recent studies have shown that fascial fibroblasts are sensitive to different stimuli (biochemical or biophysical), promoting extracellular matrix remodeling, as well as synthetic activity. Moreover, the extensive literature on the renin–angiotensin system (RAS) reported its involvement in tissue re...

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Autores principales: Pirri, Carmelo, Caroccia, Brasilina, Angelini, Andrea, Petrelli, Lucia, Piazza, Maria, Biz, Carlo, Ruggieri, Pietro, De Caro, Raffaele, Stecco, Carla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9599236/
https://www.ncbi.nlm.nih.gov/pubmed/36289870
http://dx.doi.org/10.3390/biomedicines10102608
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author Pirri, Carmelo
Caroccia, Brasilina
Angelini, Andrea
Petrelli, Lucia
Piazza, Maria
Biz, Carlo
Ruggieri, Pietro
De Caro, Raffaele
Stecco, Carla
author_facet Pirri, Carmelo
Caroccia, Brasilina
Angelini, Andrea
Petrelli, Lucia
Piazza, Maria
Biz, Carlo
Ruggieri, Pietro
De Caro, Raffaele
Stecco, Carla
author_sort Pirri, Carmelo
collection PubMed
description Recent studies have shown that fascial fibroblasts are sensitive to different stimuli (biochemical or biophysical), promoting extracellular matrix remodeling, as well as synthetic activity. Moreover, the extensive literature on the renin–angiotensin system (RAS) reported its involvement in tissue remodeling. This study aimed to investigate the presence of RAS components in the deep fascia. Thoracolumbar fascia specimens were collected from 13 patients (age range: 25–75 years; seven males and five females) who had undergone elective spinal surgical procedures at the Orthopedic Clinic of the University of Padova. Gene expression analysis was performed to investigate the expression of Ang II type 1 receptor (AT1R), Ang II type 2 receptor (AT2R), MAS receptor (MasR), angiotensinogen, angiotensin-converting enzyme 2 (ACE2) and angiotensin-converting enzyme 1 (ACE1). AT1R and ACE2 were also measured with immunoblot. AT1R was the most expressed angiotensin receptor subtype (300.2 ± 317 copies/25 ng of mRNA), followed by MasR (37.1 ± 39.56 copies/25 ng of mRNA) and AT2R (147 ± 122 copies/25 ng of mRNA). The amounts of angiotensinogen, ACE1 and ACE2 were hardly detectable. These findings demonstrate that RAS system receptors are present in the deep fascia, with a greater expression of AT1R, suggesting their involvement in fascial remodeling and fibrogenesis.
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spelling pubmed-95992362022-10-27 Evidence of Renin–Angiotensin System Receptors in Deep Fascia: A Role in Extracellular Matrix Remodeling and Fibrogenesis? Pirri, Carmelo Caroccia, Brasilina Angelini, Andrea Petrelli, Lucia Piazza, Maria Biz, Carlo Ruggieri, Pietro De Caro, Raffaele Stecco, Carla Biomedicines Article Recent studies have shown that fascial fibroblasts are sensitive to different stimuli (biochemical or biophysical), promoting extracellular matrix remodeling, as well as synthetic activity. Moreover, the extensive literature on the renin–angiotensin system (RAS) reported its involvement in tissue remodeling. This study aimed to investigate the presence of RAS components in the deep fascia. Thoracolumbar fascia specimens were collected from 13 patients (age range: 25–75 years; seven males and five females) who had undergone elective spinal surgical procedures at the Orthopedic Clinic of the University of Padova. Gene expression analysis was performed to investigate the expression of Ang II type 1 receptor (AT1R), Ang II type 2 receptor (AT2R), MAS receptor (MasR), angiotensinogen, angiotensin-converting enzyme 2 (ACE2) and angiotensin-converting enzyme 1 (ACE1). AT1R and ACE2 were also measured with immunoblot. AT1R was the most expressed angiotensin receptor subtype (300.2 ± 317 copies/25 ng of mRNA), followed by MasR (37.1 ± 39.56 copies/25 ng of mRNA) and AT2R (147 ± 122 copies/25 ng of mRNA). The amounts of angiotensinogen, ACE1 and ACE2 were hardly detectable. These findings demonstrate that RAS system receptors are present in the deep fascia, with a greater expression of AT1R, suggesting their involvement in fascial remodeling and fibrogenesis. MDPI 2022-10-17 /pmc/articles/PMC9599236/ /pubmed/36289870 http://dx.doi.org/10.3390/biomedicines10102608 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pirri, Carmelo
Caroccia, Brasilina
Angelini, Andrea
Petrelli, Lucia
Piazza, Maria
Biz, Carlo
Ruggieri, Pietro
De Caro, Raffaele
Stecco, Carla
Evidence of Renin–Angiotensin System Receptors in Deep Fascia: A Role in Extracellular Matrix Remodeling and Fibrogenesis?
title Evidence of Renin–Angiotensin System Receptors in Deep Fascia: A Role in Extracellular Matrix Remodeling and Fibrogenesis?
title_full Evidence of Renin–Angiotensin System Receptors in Deep Fascia: A Role in Extracellular Matrix Remodeling and Fibrogenesis?
title_fullStr Evidence of Renin–Angiotensin System Receptors in Deep Fascia: A Role in Extracellular Matrix Remodeling and Fibrogenesis?
title_full_unstemmed Evidence of Renin–Angiotensin System Receptors in Deep Fascia: A Role in Extracellular Matrix Remodeling and Fibrogenesis?
title_short Evidence of Renin–Angiotensin System Receptors in Deep Fascia: A Role in Extracellular Matrix Remodeling and Fibrogenesis?
title_sort evidence of renin–angiotensin system receptors in deep fascia: a role in extracellular matrix remodeling and fibrogenesis?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9599236/
https://www.ncbi.nlm.nih.gov/pubmed/36289870
http://dx.doi.org/10.3390/biomedicines10102608
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