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Radical Mediated Rapid In Vitro Formation of c-Type Cytochrome

A cytochrome c(552) mutant from Thermus thermophilus HB8 (rC(552) C14A) was reported, where the polypeptide with replaced Cys14 by alanine, overexpressed in the cytosol of E. coli. The apo-form of the C14A mutant (apo-C14A) without the original prosthetic group was obtained by simple chemical treatm...

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Detalles Bibliográficos
Autores principales: Ibrahim, Sheikh Muhammad, Ben Aoun, Sami, Nakajima, Hiroshi, Kooli, Fethi, Watanabe, Yoshihito
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9599503/
https://www.ncbi.nlm.nih.gov/pubmed/36291538
http://dx.doi.org/10.3390/biom12101329