Circular Whole-Transcriptome Amplification (cWTA) and mNGS Screening Enhanced by a Group Testing Algorithm (mEGA) Enable High-Throughput and Comprehensive Virus Identification

Metagenomic next-generation sequencing (mNGS) offers a hypothesis-free approach for pathogen detection, but its applicability in clinical diagnosis, in addition to other factors, remains limited due to complicated library construction. The present study describes a PCR-free isothermal workflow for m...

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Detalles Bibliográficos
Autores principales: Reteng, Patrick, Nguyen Thuy, Linh, Rahman, Mizanur, Bispo de Filippis, Ana Maria, Hayashida, Kyoko, Sugi, Tatsuki, Gonzalez, Gabriel, Hall, William W., Nguyen Thi, Lan Anh, Yamagishi, Junya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9599668/
https://www.ncbi.nlm.nih.gov/pubmed/36005385
http://dx.doi.org/10.1128/msphere.00332-22

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9599668/
https://www.ncbi.nlm.nih.gov/pubmed/36005385
http://dx.doi.org/10.1128/msphere.00332-22

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