The Highly Efficient Expression System of Recombinant Human Prolidase and the Effect of N-Terminal His-Tag on the Enzyme Activity

Prolidase is an enzyme hydrolyzing dipeptides containing proline or hydroxyprolineat the C-terminus and plays an important role in collagen turnover. Human prolidase is active as a dimer with the C-terminal domain containing two Mn(2+) ions in its active site. The study aimed to develop a highly eff...

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Detalles Bibliográficos
Autores principales: Czyrko-Horczak, Justyna, Nizioł, Magdalena, Forlino, Antonella, Besio, Roberta, Miltyk, Wojciech
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9600000/
https://www.ncbi.nlm.nih.gov/pubmed/36291150
http://dx.doi.org/10.3390/cells11203284

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9600000/
https://www.ncbi.nlm.nih.gov/pubmed/36291150
http://dx.doi.org/10.3390/cells11203284

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