Cargando…
Structure determination of an unstable macromolecular complex enabled by nanobody‐peptide bridging
Structure determination of macromolecular complexes is challenging if subunits can dissociate during crystallization or preparation of electron microscopy grids. We present an approach where a labile complex is stabilized by linking subunits though introduction of a peptide tag in one subunit that i...
| Autores principales: | , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley & Sons, Inc.
2022
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9601772/ https://www.ncbi.nlm.nih.gov/pubmed/36173177 http://dx.doi.org/10.1002/pro.4432 |