Flow-Seq Evaluation of Translation Driven by a Set of Natural Escherichia coli 5′-UTR of Variable Length

Flow-seq is a method that combines fluorescently activated cell sorting and next-generation sequencing to deduce a large amount of data about translation efficiency from a single experiment. Here, we constructed a library of fluorescent protein-based reporters preceded by a set of 648 natural 5′-unt...

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Detalles Bibliográficos
Autores principales: Komarova, Ekaterina S., Slesarchuk, Anna N., Rubtsova, Maria P., Osterman, Ilya A., Tupikin, Alexey E., Pyshnyi, Dmitry V., Dontsova, Olga A., Kabilov, Marsel R., Sergiev, Petr V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9604319/
https://www.ncbi.nlm.nih.gov/pubmed/36293163
http://dx.doi.org/10.3390/ijms232012293

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9604319/
https://www.ncbi.nlm.nih.gov/pubmed/36293163
http://dx.doi.org/10.3390/ijms232012293

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