Cargando…

Flow-Seq Evaluation of Translation Driven by a Set of Natural Escherichia coli 5′-UTR of Variable Length

Flow-seq is a method that combines fluorescently activated cell sorting and next-generation sequencing to deduce a large amount of data about translation efficiency from a single experiment. Here, we constructed a library of fluorescent protein-based reporters preceded by a set of 648 natural 5′-unt...

Descripción completa

Detalles Bibliográficos
Autores principales:	Komarova, Ekaterina S., Slesarchuk, Anna N., Rubtsova, Maria P., Osterman, Ilya A., Tupikin, Alexey E., Pyshnyi, Dmitry V., Dontsova, Olga A., Kabilov, Marsel R., Sergiev, Petr V.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9604319/ https://www.ncbi.nlm.nih.gov/pubmed/36293163 http://dx.doi.org/10.3390/ijms232012293

Ejemplares similares

Flow-Seq Method: Features and Application in Bacterial Translation Studies
por: Komarova, E. S., et al.
Publicado: (2022)

Application of sorting and next generation sequencing to study 5΄-UTR influence on translation efficiency in Escherichia coli
por: Evfratov, Sergey A., et al.
Publicado: (2017)

Translation at first sight: the influence of leading codons
por: Osterman, Ilya A, et al.
Publicado: (2020)

Influence of the spacer region between the Shine–Dalgarno box and the start codon for fine‐tuning of the translation efficiency in Escherichia coli
por: Komarova, Ekaterina S., et al.
Publicado: (2020)

Techniques for Screening Translation Inhibitors
por: Osterman, Ilya A., et al.
Publicado: (2016)

Comprehensive Functional Analysis of Escherichia coli Ribosomal RNA Methyltransferases
por: Pletnev, Philipp, et al.
Publicado: (2020)

Madumycin II inhibits peptide bond formation by forcing the peptidyl transferase center into an inactive state
por: Osterman, Ilya A., et al.
Publicado: (2017)

Comparison of mRNA features affecting translation initiation and reinitiation
por: Osterman, Ilya A., et al.
Publicado: (2013)

Survival guide: Escherichia coli in the stationary phase
por: Pletnev, P., et al.
Publicado: (2015)

Draft Genome Sequence of the Probiotic Bifidobacterium longum subsp. longum Strain MC-42
por: Tupikin, Alexey E., et al.
Publicado: (2016)

Modified nucleotides m(2)G966/m(5)C967 of Escherichia coli 16S rRNA are required for attenuation of tryptophan operon
por: Prokhorova, Irina V., et al.
Publicado: (2013)

Mutation at the Site of Hydroxylation in the Ribosomal Protein uL15 (RPL27a) Causes Specific Changes in the Repertoire of mRNAs Translated in Mammalian Cells
por: Zolotenkova, Elizaveta A., et al.
Publicado: (2023)

How much can we learn about the function of bacterial rRNA modification by mining large-scale experimental datasets?
por: Sergiev, Petr V., et al.
Publicado: (2012)

Escherichia coli ItaT is a type II toxin that inhibits translation by acetylating isoleucyl-tRNA(Ile)
por: Wilcox, Brendan, et al.
Publicado: (2018)

Knockdown of the Ribosomal Protein eL38 in HEK293 Cells Changes the Translational Efficiency of Specific Genes
por: Gopanenko, Alexander V., et al.
Publicado: (2021)

Deficiency of the Ribosomal Protein uL5 Leads to Significant Rearrangements of the Transcriptional and Translational Landscapes in Mammalian Cells
por: Babaylova, Elena S., et al.
Publicado: (2021)

METTL15 interacts with the assembly intermediate of murine mitochondrial small ribosomal subunit to form m(4)C840 12S rRNA residue
por: Laptev, Ivan, et al.
Publicado: (2020)

Posttranslational Modifications of Ribosomal Proteins in Escherichia coli
por: Nesterchuk, M.V., et al.
Publicado: (2011)

Extended Evaluation of Viral Diversity in Lake Baikal through Metagenomics
por: Butina, Tatyana V., et al.
Publicado: (2021)

Insights into the improved macrolide inhibitory activity from the high-resolution cryo-EM structure of dirithromycin bound to the E. coli 70S ribosome
por: Pichkur, Evgeny B., et al.
Publicado: (2020)

Rewiring of growth-dependent transcription regulation by a point mutation in region 1.1 of the housekeeping σ factor
por: Pletnev, Philipp, et al.
Publicado: (2020)

Human ribosomal protein eS1 is engaged in cellular events related to processing and functioning of U11 snRNA
por: Gopanenko, Alexander V., et al.
Publicado: (2017)

Ribosomal RNA guanine-(N2)-methyltransferases and their targets
por: Sergiev, Petr V., et al.
Publicado: (2007)

Changes in the Transcriptome Caused by Mutations in the Ribosomal Protein uS10 Associated with a Predisposition to Colorectal Cancer
por: Tian, Yueming, et al.
Publicado: (2022)

RtcB2-PrfH Operon Protects E. coli ATCC25922 Strain from Colicin E3 Toxin
por: Maviza, Tinashe P., et al.
Publicado: (2022)

Recognition but no repair of abasic site in single-stranded DNA by human ribosomal uS3 protein residing within intact 40S subunit
por: Grosheva, Anastasia S., et al.
Publicado: (2017)

mRNA regions where 80S ribosomes pause during translation elongation in vivo interact with protein uS19, a component of the decoding site
por: Babaylova, Elena S, et al.
Publicado: (2020)

Reorganization of the Landscape of Translated mRNAs in NSUN2-Deficient Cells and Specific Features of NSUN2 Target mRNAs
por: Kossinova, Olga A., et al.
Publicado: (2022)

Identification of Key Genes and Pathways in Genotoxic Stress Induced Endothelial Dysfunction: Results of Whole Transcriptome Sequencing
por: Sinitsky, Maxim, et al.
Publicado: (2022)

Complete Genome Sequence of a Western Siberian Lymantria dispar Multiple Nucleopolyhedrovirus Isolate
por: Kabilov, Marsel R., et al.
Publicado: (2015)

Amicoumacin A induces cancer cell death by targeting the eukaryotic ribosome
por: Prokhorova, Irina V., et al.
Publicado: (2016)

Knockdown of the Ribosomal Protein eL29 in Mammalian Cells Leads to Significant Changes in Gene Expression at the Transcription Level
por: Gopanenko, Alexander V., et al.
Publicado: (2020)

Human Peripheral Blood-Derived Endothelial Colony-Forming Cells Are Highly Similar to Mature Vascular Endothelial Cells yet Demonstrate a Transitional Transcriptomic Signature
por: Kutikhin, Anton G., et al.
Publicado: (2020)

Reporter-recruiting bifunctional aptasensor for bioluminescent analytical assays
por: Davydova, Anna, et al.
Publicado: (2020)

Comparative Metagenomic Profiling of Symbiotic Bacterial Communities Associated with Ixodes persulcatus, Ixodes pavlovskyi and Dermacentor reticulatus Ticks
por: Kurilshikov, Alexander, et al.
Publicado: (2015)

Studies on Antibacterial Activity and Diversity of Cultivable Actinobacteria Isolated from Mangrove Soil in Futian and Maoweihai of China
por: Li, Feina, et al.
Publicado: (2019)

Multi-Omics Profiling of Human Endothelial Cells from the Coronary Artery and Internal Thoracic Artery Reveals Molecular but Not Functional Heterogeneity
por: Frolov, Alexey, et al.
Publicado: (2023)

Epitranscriptomics of Mammalian Mitochondrial Ribosomal RNA
por: Laptev, Ivan, et al.
Publicado: (2020)

Multifaceted Mechanism of Amicoumacin A Inhibition of Bacterial Translation
por: Maksimova, Elena M., et al.
Publicado: (2021)

Complete Genome Sequencing of Borrelia valaisiana and Borrelia afzelii Isolated from Ixodes persulcatus Ticks in Western Siberia
por: Kurilshikov, Alexander M., et al.
Publicado: (2014)

Cannot write session to /tmp/vufind_sessions/sess_npsku8p6k0mp04eno3tv8gddm3