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Whole Genome Sequencing Analysis of Effects of CRISPR/Cas9 in Komagataella phaffii: A Budding Yeast in Distress

The industrially important non-conventional yeast Komagataella phaffii suffers from low rates of homologous recombination, making site specific genetic engineering tedious. Therefore, genome editing using CRISPR/Cas represents a simple and efficient alternative. To characterize on- and off-target mu...

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Detalles Bibliográficos
Autores principales: Schusterbauer, Veronika, Fischer, Jasmin E., Gangl, Sarah, Schenzle, Lisa, Rinnofner, Claudia, Geier, Martina, Sailer, Christian, Glieder, Anton, Thallinger, Gerhard G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9605565/
https://www.ncbi.nlm.nih.gov/pubmed/36294556
http://dx.doi.org/10.3390/jof8100992