CasPlay provides a gRNA-barcoded CRISPR-based display platform for antibody repertoire profiling

Protein display technologies link proteins to distinct nucleic acid sequences (barcodes), enabling multiplexed protein assays via DNA sequencing. Here, we develop Cas9 display (CasPlay) to interrogate customized peptide libraries fused to catalytically inactive Cas9 (dCas9) by sequencing the guide R...

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Detalles Bibliográficos
Autores principales: Barber, Karl W., Shrock, Ellen, Elledge, Stephen J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9606310/
https://www.ncbi.nlm.nih.gov/pubmed/36313802
http://dx.doi.org/10.1016/j.crmeth.2022.100318

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9606310/
https://www.ncbi.nlm.nih.gov/pubmed/36313802
http://dx.doi.org/10.1016/j.crmeth.2022.100318

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