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Optimising expression of the large dynamic range FRET pair mNeonGreen and superfolder mTurquoise2(ox) for use in the Escherichia coli cytoplasm

The fluorescent proteins superfolder mTurquoise2(ox) (sfTq2(ox)) and mNeonGreen function excellently in mammalian cells, but are not well expressed in E. coli when forming the N-terminus of constructs. Expression was increased by decreasing structures at the start of their coding sequences in the mR...

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Detalles Bibliográficos
Autores principales: Mertens, Laureen M. Y., den Blaauwen, Tanneke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9606377/
https://www.ncbi.nlm.nih.gov/pubmed/36289441
http://dx.doi.org/10.1038/s41598-022-22918-2