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Optimising expression of the large dynamic range FRET pair mNeonGreen and superfolder mTurquoise2(ox) for use in the Escherichia coli cytoplasm
The fluorescent proteins superfolder mTurquoise2(ox) (sfTq2(ox)) and mNeonGreen function excellently in mammalian cells, but are not well expressed in E. coli when forming the N-terminus of constructs. Expression was increased by decreasing structures at the start of their coding sequences in the mR...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group UK
2022
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9606377/ https://www.ncbi.nlm.nih.gov/pubmed/36289441 http://dx.doi.org/10.1038/s41598-022-22918-2 |