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Simple and efficient protocol to isolate and culture brain microvascular endothelial cells from newborn mice

The neurovascular unit (NVU) is a multicellular structure comprising of neurons, glial cells, and non-neural cells, and it is supported by a specialized extracellular matrix, the basal lamina. Astrocytes, brain microvascular endothelial cells (BMECs), pericytes, and smooth muscle cells constitute th...

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Detalles Bibliográficos
Autores principales: Nicolicht-Amorim, Priscila, Delgado-Garcia, Lina M., Nakamura, Thabatta Karollynne Estevam, Courbassier, Natália Rodrigues, Mosini, Amanda Cristina, Porcionatto, Marimelia A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9606660/
https://www.ncbi.nlm.nih.gov/pubmed/36313615
http://dx.doi.org/10.3389/fncel.2022.949412