Microbiome of Citrullus colocynthis (L.) Schrad. Reveals a Potential Association with Non-Photosynthetic Cyanobacteria

Citrullus colocynthis grows in the sandy desert soil of the Arabian Peninsula with limited access to water, aside from occasional precipitation or dew. Understanding its ability to produce water-filled fruit and nutrient-rich seeds despite the harsh environment, can be useful for agricultural applic...

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Autores principales: Procter, Miranda, Kundu, Biduth, Sudalaimuthuasari, Naganeeswaran, AlMaskari, Raja S., Saeed, Esam E., Hazzouri, Khaled M., Amiri, Khaled M. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607294/
https://www.ncbi.nlm.nih.gov/pubmed/36296358
http://dx.doi.org/10.3390/microorganisms10102083
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author Procter, Miranda
Kundu, Biduth
Sudalaimuthuasari, Naganeeswaran
AlMaskari, Raja S.
Saeed, Esam E.
Hazzouri, Khaled M.
Amiri, Khaled M. A.
author_facet Procter, Miranda
Kundu, Biduth
Sudalaimuthuasari, Naganeeswaran
AlMaskari, Raja S.
Saeed, Esam E.
Hazzouri, Khaled M.
Amiri, Khaled M. A.
author_sort Procter, Miranda
collection PubMed
description Citrullus colocynthis grows in the sandy desert soil of the Arabian Peninsula with limited access to water, aside from occasional precipitation or dew. Understanding its ability to produce water-filled fruit and nutrient-rich seeds despite the harsh environment, can be useful for agricultural applications. However, information regarding the microbiome of C. colocynthis is lacking. We hypothesized that C. colocynthis associates with bacteria that aid its survival, like what has been observed in other desert plants. Here, we used 16S rRNA gene data to gain insight into the microbiome of C. colocynthis to identify its associated bacteria. In total, 9818 and 6983 OTUs were generated from root, soil, and leaf samples combined. Overall, bulk soils had the highest alpha diversity, followed by rhizosphere and root zone soils. Furthermore, C. colocynthis is associated with known plant-growth-promoting bacteria (including Acidobacteria, Bacterioidetes, and Actinobacteria), and interestingly a class of non-photosynthetic Cyanobacteria (Melainabacteria) that is more abundant on the inside and outside of the root surface than control samples, suggesting its involvement in the rhizophagy process. This study will provide a foundation for functional studies to further understand how C. colocynthis-microbes interactions help them grow in the desert, paving the path for possible agricultural applications.
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spelling pubmed-96072942022-10-28 Microbiome of Citrullus colocynthis (L.) Schrad. Reveals a Potential Association with Non-Photosynthetic Cyanobacteria Procter, Miranda Kundu, Biduth Sudalaimuthuasari, Naganeeswaran AlMaskari, Raja S. Saeed, Esam E. Hazzouri, Khaled M. Amiri, Khaled M. A. Microorganisms Article Citrullus colocynthis grows in the sandy desert soil of the Arabian Peninsula with limited access to water, aside from occasional precipitation or dew. Understanding its ability to produce water-filled fruit and nutrient-rich seeds despite the harsh environment, can be useful for agricultural applications. However, information regarding the microbiome of C. colocynthis is lacking. We hypothesized that C. colocynthis associates with bacteria that aid its survival, like what has been observed in other desert plants. Here, we used 16S rRNA gene data to gain insight into the microbiome of C. colocynthis to identify its associated bacteria. In total, 9818 and 6983 OTUs were generated from root, soil, and leaf samples combined. Overall, bulk soils had the highest alpha diversity, followed by rhizosphere and root zone soils. Furthermore, C. colocynthis is associated with known plant-growth-promoting bacteria (including Acidobacteria, Bacterioidetes, and Actinobacteria), and interestingly a class of non-photosynthetic Cyanobacteria (Melainabacteria) that is more abundant on the inside and outside of the root surface than control samples, suggesting its involvement in the rhizophagy process. This study will provide a foundation for functional studies to further understand how C. colocynthis-microbes interactions help them grow in the desert, paving the path for possible agricultural applications. MDPI 2022-10-21 /pmc/articles/PMC9607294/ /pubmed/36296358 http://dx.doi.org/10.3390/microorganisms10102083 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Procter, Miranda
Kundu, Biduth
Sudalaimuthuasari, Naganeeswaran
AlMaskari, Raja S.
Saeed, Esam E.
Hazzouri, Khaled M.
Amiri, Khaled M. A.
Microbiome of Citrullus colocynthis (L.) Schrad. Reveals a Potential Association with Non-Photosynthetic Cyanobacteria
title Microbiome of Citrullus colocynthis (L.) Schrad. Reveals a Potential Association with Non-Photosynthetic Cyanobacteria
title_full Microbiome of Citrullus colocynthis (L.) Schrad. Reveals a Potential Association with Non-Photosynthetic Cyanobacteria
title_fullStr Microbiome of Citrullus colocynthis (L.) Schrad. Reveals a Potential Association with Non-Photosynthetic Cyanobacteria
title_full_unstemmed Microbiome of Citrullus colocynthis (L.) Schrad. Reveals a Potential Association with Non-Photosynthetic Cyanobacteria
title_short Microbiome of Citrullus colocynthis (L.) Schrad. Reveals a Potential Association with Non-Photosynthetic Cyanobacteria
title_sort microbiome of citrullus colocynthis (l.) schrad. reveals a potential association with non-photosynthetic cyanobacteria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607294/
https://www.ncbi.nlm.nih.gov/pubmed/36296358
http://dx.doi.org/10.3390/microorganisms10102083
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