A Simple, Improved Method for Scarless Genome Editing of Budding Yeast Using CRISPR-Cas9

Until recently, the favored method for making directed modifications to the budding yeast genome involved the introduction of a DNA template carrying the desired genetic changes along with a selectable marker, flanked by homology arms. This approach both limited the ability to make changes within ge...

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Detalles Bibliográficos
Autores principales: Aguilar, Rhiannon R., Shen, Zih-Jie, Tyler, Jessica K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607540/
https://www.ncbi.nlm.nih.gov/pubmed/36287051
http://dx.doi.org/10.3390/mps5050079

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607540/
https://www.ncbi.nlm.nih.gov/pubmed/36287051
http://dx.doi.org/10.3390/mps5050079

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