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A Simple, Improved Method for Scarless Genome Editing of Budding Yeast Using CRISPR-Cas9
Until recently, the favored method for making directed modifications to the budding yeast genome involved the introduction of a DNA template carrying the desired genetic changes along with a selectable marker, flanked by homology arms. This approach both limited the ability to make changes within ge...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
MDPI
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607540/ https://www.ncbi.nlm.nih.gov/pubmed/36287051 http://dx.doi.org/10.3390/mps5050079 |