Biochemical characterization of a cyanobactin arginine-N-prenylase from the autumnalamide biosynthetic pathway

Cyanobactins are linear and cyclic post-translationally modified peptides. Here we show that the prenyl-d-Arg-containing autumnalamide A is a member of the cyanobactin family. Biochemical assays demonstrate that the AutF prenyltransferase targets the guanidinium moiety in arginine and homoarginine a...

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Autores principales: Clemente, Claudia, Johnson, Nicholas, Ouyang, Xiaodan, Popin, Rafael V., Dall'Angelo, Sergio, Wahlsten, Matti, Jokela, Jouni, Colombano, Alessandro, Nardone, Brunello, Fewer, David P., Houssen, Wael E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9609003/
https://www.ncbi.nlm.nih.gov/pubmed/36193595
http://dx.doi.org/10.1039/d2cc01799g
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author Clemente, Claudia
Johnson, Nicholas
Ouyang, Xiaodan
Popin, Rafael V.
Dall'Angelo, Sergio
Wahlsten, Matti
Jokela, Jouni
Colombano, Alessandro
Nardone, Brunello
Fewer, David P.
Houssen, Wael E.
author_facet Clemente, Claudia
Johnson, Nicholas
Ouyang, Xiaodan
Popin, Rafael V.
Dall'Angelo, Sergio
Wahlsten, Matti
Jokela, Jouni
Colombano, Alessandro
Nardone, Brunello
Fewer, David P.
Houssen, Wael E.
author_sort Clemente, Claudia
collection PubMed
description Cyanobactins are linear and cyclic post-translationally modified peptides. Here we show that the prenyl-d-Arg-containing autumnalamide A is a member of the cyanobactin family. Biochemical assays demonstrate that the AutF prenyltransferase targets the guanidinium moiety in arginine and homoarginine and is a useful tool for biotechnological applications.
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spelling pubmed-96090032022-11-07 Biochemical characterization of a cyanobactin arginine-N-prenylase from the autumnalamide biosynthetic pathway Clemente, Claudia Johnson, Nicholas Ouyang, Xiaodan Popin, Rafael V. Dall'Angelo, Sergio Wahlsten, Matti Jokela, Jouni Colombano, Alessandro Nardone, Brunello Fewer, David P. Houssen, Wael E. Chem Commun (Camb) Chemistry Cyanobactins are linear and cyclic post-translationally modified peptides. Here we show that the prenyl-d-Arg-containing autumnalamide A is a member of the cyanobactin family. Biochemical assays demonstrate that the AutF prenyltransferase targets the guanidinium moiety in arginine and homoarginine and is a useful tool for biotechnological applications. The Royal Society of Chemistry 2022-09-28 /pmc/articles/PMC9609003/ /pubmed/36193595 http://dx.doi.org/10.1039/d2cc01799g Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Clemente, Claudia
Johnson, Nicholas
Ouyang, Xiaodan
Popin, Rafael V.
Dall'Angelo, Sergio
Wahlsten, Matti
Jokela, Jouni
Colombano, Alessandro
Nardone, Brunello
Fewer, David P.
Houssen, Wael E.
Biochemical characterization of a cyanobactin arginine-N-prenylase from the autumnalamide biosynthetic pathway
title Biochemical characterization of a cyanobactin arginine-N-prenylase from the autumnalamide biosynthetic pathway
title_full Biochemical characterization of a cyanobactin arginine-N-prenylase from the autumnalamide biosynthetic pathway
title_fullStr Biochemical characterization of a cyanobactin arginine-N-prenylase from the autumnalamide biosynthetic pathway
title_full_unstemmed Biochemical characterization of a cyanobactin arginine-N-prenylase from the autumnalamide biosynthetic pathway
title_short Biochemical characterization of a cyanobactin arginine-N-prenylase from the autumnalamide biosynthetic pathway
title_sort biochemical characterization of a cyanobactin arginine-n-prenylase from the autumnalamide biosynthetic pathway
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9609003/
https://www.ncbi.nlm.nih.gov/pubmed/36193595
http://dx.doi.org/10.1039/d2cc01799g
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