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Enhanced Production of ECM Proteins for Pharmaceutical Applications Using Mammalian Cells and Sodium Heparin Supplementation
The yields of soluble ECM proteins recombinantly produced with mammalian cells can be significantly enhanced by exploiting the stabilizing properties of heparin. Here, we propose a simple and straightforward scalable protocol for the mammalian cell production of ECM proteins with affinity for hepari...
Autores principales: | Garcia-Pardo, Javier, Montané, Sergi, Avilés, Francesc Xavier, Tanco, Sebastian, Lorenzo, Julia |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9609459/ https://www.ncbi.nlm.nih.gov/pubmed/36297573 http://dx.doi.org/10.3390/pharmaceutics14102138 |
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