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Locust Bean Gum Nano-Based Hydrogel for Vaginal Delivery of Diphenyl Diselenide in the Treatment of Trichomoniasis: Formulation Characterization and In Vitro Biological Evaluation

Trichomoniasis is the most common nonviral sexually transmitted infection in the world, but its available therapies present low efficacy and high toxicity. Diphenyl diselenide (PhSe(2)) is a pharmacologically active organic selenium compound; however, its clinical use is hindered by its lipophilicit...

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Autores principales: dos Reis, Fernanda Padoin, Rigo, Graziela Vargas, Nogueira, Cristina Wayne, Tasca, Tiana, Sari, Marcel Henrique Marcondes, Cruz, Letícia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9610892/
https://www.ncbi.nlm.nih.gov/pubmed/36297547
http://dx.doi.org/10.3390/pharmaceutics14102112
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author dos Reis, Fernanda Padoin
Rigo, Graziela Vargas
Nogueira, Cristina Wayne
Tasca, Tiana
Sari, Marcel Henrique Marcondes
Cruz, Letícia
author_facet dos Reis, Fernanda Padoin
Rigo, Graziela Vargas
Nogueira, Cristina Wayne
Tasca, Tiana
Sari, Marcel Henrique Marcondes
Cruz, Letícia
author_sort dos Reis, Fernanda Padoin
collection PubMed
description Trichomoniasis is the most common nonviral sexually transmitted infection in the world, but its available therapies present low efficacy and high toxicity. Diphenyl diselenide (PhSe(2)) is a pharmacologically active organic selenium compound; however, its clinical use is hindered by its lipophilicity and toxicity. Nanocarriers are an interesting approach to overcome the limitations associated with this compound. This study designed and evaluated a vaginal hydrogel containing PhSe(2)-loaded Eudragit(®) RS100 and coconut oil nanocapsules for the treatment of trichomoniasis. Nanocapsules presented particle sizes in the nanometric range, positive zeta potential, a compound content close to the theoretical value, and high encapsulation efficiency. The nanoencapsulation maintained the anti-Trichomonas vaginalis action of the compound while improving the scavenger action in a DPPH assay. The hydrogels were prepared by thickening nanocapsule suspensions with locust bean gum (3%). The semisolids maintained the nanometric size of the particles and the PhSe(2) content at around the initial concentration (1.0 mg/g). They also displayed non-Newtonian pseudo-plastic behavior and a highly mucoadhesive property. The chorioallantoic membrane method indicated the absence of hemorrhage, coagulation, or lysis. The compound, from both non-encapsulated and nano-based hydrogel delivery systems, remained on the surface of the bovine vaginal mucosa. Therefore, the formulations displayed the intended properties and could be a promising alternative for the treatment of trichomoniasis.
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spelling pubmed-96108922022-10-28 Locust Bean Gum Nano-Based Hydrogel for Vaginal Delivery of Diphenyl Diselenide in the Treatment of Trichomoniasis: Formulation Characterization and In Vitro Biological Evaluation dos Reis, Fernanda Padoin Rigo, Graziela Vargas Nogueira, Cristina Wayne Tasca, Tiana Sari, Marcel Henrique Marcondes Cruz, Letícia Pharmaceutics Article Trichomoniasis is the most common nonviral sexually transmitted infection in the world, but its available therapies present low efficacy and high toxicity. Diphenyl diselenide (PhSe(2)) is a pharmacologically active organic selenium compound; however, its clinical use is hindered by its lipophilicity and toxicity. Nanocarriers are an interesting approach to overcome the limitations associated with this compound. This study designed and evaluated a vaginal hydrogel containing PhSe(2)-loaded Eudragit(®) RS100 and coconut oil nanocapsules for the treatment of trichomoniasis. Nanocapsules presented particle sizes in the nanometric range, positive zeta potential, a compound content close to the theoretical value, and high encapsulation efficiency. The nanoencapsulation maintained the anti-Trichomonas vaginalis action of the compound while improving the scavenger action in a DPPH assay. The hydrogels were prepared by thickening nanocapsule suspensions with locust bean gum (3%). The semisolids maintained the nanometric size of the particles and the PhSe(2) content at around the initial concentration (1.0 mg/g). They also displayed non-Newtonian pseudo-plastic behavior and a highly mucoadhesive property. The chorioallantoic membrane method indicated the absence of hemorrhage, coagulation, or lysis. The compound, from both non-encapsulated and nano-based hydrogel delivery systems, remained on the surface of the bovine vaginal mucosa. Therefore, the formulations displayed the intended properties and could be a promising alternative for the treatment of trichomoniasis. MDPI 2022-10-03 /pmc/articles/PMC9610892/ /pubmed/36297547 http://dx.doi.org/10.3390/pharmaceutics14102112 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
dos Reis, Fernanda Padoin
Rigo, Graziela Vargas
Nogueira, Cristina Wayne
Tasca, Tiana
Sari, Marcel Henrique Marcondes
Cruz, Letícia
Locust Bean Gum Nano-Based Hydrogel for Vaginal Delivery of Diphenyl Diselenide in the Treatment of Trichomoniasis: Formulation Characterization and In Vitro Biological Evaluation
title Locust Bean Gum Nano-Based Hydrogel for Vaginal Delivery of Diphenyl Diselenide in the Treatment of Trichomoniasis: Formulation Characterization and In Vitro Biological Evaluation
title_full Locust Bean Gum Nano-Based Hydrogel for Vaginal Delivery of Diphenyl Diselenide in the Treatment of Trichomoniasis: Formulation Characterization and In Vitro Biological Evaluation
title_fullStr Locust Bean Gum Nano-Based Hydrogel for Vaginal Delivery of Diphenyl Diselenide in the Treatment of Trichomoniasis: Formulation Characterization and In Vitro Biological Evaluation
title_full_unstemmed Locust Bean Gum Nano-Based Hydrogel for Vaginal Delivery of Diphenyl Diselenide in the Treatment of Trichomoniasis: Formulation Characterization and In Vitro Biological Evaluation
title_short Locust Bean Gum Nano-Based Hydrogel for Vaginal Delivery of Diphenyl Diselenide in the Treatment of Trichomoniasis: Formulation Characterization and In Vitro Biological Evaluation
title_sort locust bean gum nano-based hydrogel for vaginal delivery of diphenyl diselenide in the treatment of trichomoniasis: formulation characterization and in vitro biological evaluation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9610892/
https://www.ncbi.nlm.nih.gov/pubmed/36297547
http://dx.doi.org/10.3390/pharmaceutics14102112
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