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Simultaneous Determination of Glibenclamide and Silymarin Released from Chitosan Microparticles by HPLC-ESI-MS Technique: Method Development and Validation

The study aim was to develop and validate a high-performance liquid chromatography–electrospray ionization mass spectrometry (HPLC-ESI-MS) method to simultaneously determine glibenclamide (Gli) and silymarin (Sil) released from chitosan (CS) microparticles in aqueous solutions. The CS microparticles...

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Autores principales: Condurache, Mihaela-Iustina, Petrovici, Anca-Roxana, Simionescu, Natalia, Profire, Bianca-Stefania, Confederat, Luminita-Georgeta, Bujor, Alexandra, Miron, Anca, Profire, Lenuta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9611085/
https://www.ncbi.nlm.nih.gov/pubmed/36297600
http://dx.doi.org/10.3390/pharmaceutics14102164
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author Condurache, Mihaela-Iustina
Petrovici, Anca-Roxana
Simionescu, Natalia
Profire, Bianca-Stefania
Confederat, Luminita-Georgeta
Bujor, Alexandra
Miron, Anca
Profire, Lenuta
author_facet Condurache, Mihaela-Iustina
Petrovici, Anca-Roxana
Simionescu, Natalia
Profire, Bianca-Stefania
Confederat, Luminita-Georgeta
Bujor, Alexandra
Miron, Anca
Profire, Lenuta
author_sort Condurache, Mihaela-Iustina
collection PubMed
description The study aim was to develop and validate a high-performance liquid chromatography–electrospray ionization mass spectrometry (HPLC-ESI-MS) method to simultaneously determine glibenclamide (Gli) and silymarin (Sil) released from chitosan (CS) microparticles in aqueous solutions. The CS microparticles were synthesized using an ionic gelation method, and their morphology, swelling degree, encapsulation efficiency and active substance release were investigated. Gli and Sil were loaded in different concentrations, and their identification and quantification were performed using the HPLC-ESI-MS method, which was further validated. The drugs’ characteristic m/z was found in the higher intensity of retention time (Rt) (Gli, 8.909 min; Sil A, 5.41 min; and Sil B, 5.66 min). The method selectivity and precision are very good, and the blank solution proved no interference. The linearity of the answer function is very good for Sil A (R(2) = 1), Sil B (R(2) = 0.9998) and Gli (R(2) = 0.9991). For Gli, we obtained a limit of detection (LOD) = 0.038 mg/mL and limit of quantification (LOQ) = 1.275 mg/mL; for Sil A, a LOD = 0.285 mg/mL and LOQ = 0.95 mg/mL; and for Sil B, a LOD = 0.045 mg/mL and LOQ = 0.15 mg/mL. A high-resolution HPLC-ESI-MS method was developed and validated, which allowed the simultaneous determination of Gli and Sil loaded in CS microparticles, in a concentration range of 0.025–1 mg/mL.
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spelling pubmed-96110852022-10-28 Simultaneous Determination of Glibenclamide and Silymarin Released from Chitosan Microparticles by HPLC-ESI-MS Technique: Method Development and Validation Condurache, Mihaela-Iustina Petrovici, Anca-Roxana Simionescu, Natalia Profire, Bianca-Stefania Confederat, Luminita-Georgeta Bujor, Alexandra Miron, Anca Profire, Lenuta Pharmaceutics Article The study aim was to develop and validate a high-performance liquid chromatography–electrospray ionization mass spectrometry (HPLC-ESI-MS) method to simultaneously determine glibenclamide (Gli) and silymarin (Sil) released from chitosan (CS) microparticles in aqueous solutions. The CS microparticles were synthesized using an ionic gelation method, and their morphology, swelling degree, encapsulation efficiency and active substance release were investigated. Gli and Sil were loaded in different concentrations, and their identification and quantification were performed using the HPLC-ESI-MS method, which was further validated. The drugs’ characteristic m/z was found in the higher intensity of retention time (Rt) (Gli, 8.909 min; Sil A, 5.41 min; and Sil B, 5.66 min). The method selectivity and precision are very good, and the blank solution proved no interference. The linearity of the answer function is very good for Sil A (R(2) = 1), Sil B (R(2) = 0.9998) and Gli (R(2) = 0.9991). For Gli, we obtained a limit of detection (LOD) = 0.038 mg/mL and limit of quantification (LOQ) = 1.275 mg/mL; for Sil A, a LOD = 0.285 mg/mL and LOQ = 0.95 mg/mL; and for Sil B, a LOD = 0.045 mg/mL and LOQ = 0.15 mg/mL. A high-resolution HPLC-ESI-MS method was developed and validated, which allowed the simultaneous determination of Gli and Sil loaded in CS microparticles, in a concentration range of 0.025–1 mg/mL. MDPI 2022-10-11 /pmc/articles/PMC9611085/ /pubmed/36297600 http://dx.doi.org/10.3390/pharmaceutics14102164 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Condurache, Mihaela-Iustina
Petrovici, Anca-Roxana
Simionescu, Natalia
Profire, Bianca-Stefania
Confederat, Luminita-Georgeta
Bujor, Alexandra
Miron, Anca
Profire, Lenuta
Simultaneous Determination of Glibenclamide and Silymarin Released from Chitosan Microparticles by HPLC-ESI-MS Technique: Method Development and Validation
title Simultaneous Determination of Glibenclamide and Silymarin Released from Chitosan Microparticles by HPLC-ESI-MS Technique: Method Development and Validation
title_full Simultaneous Determination of Glibenclamide and Silymarin Released from Chitosan Microparticles by HPLC-ESI-MS Technique: Method Development and Validation
title_fullStr Simultaneous Determination of Glibenclamide and Silymarin Released from Chitosan Microparticles by HPLC-ESI-MS Technique: Method Development and Validation
title_full_unstemmed Simultaneous Determination of Glibenclamide and Silymarin Released from Chitosan Microparticles by HPLC-ESI-MS Technique: Method Development and Validation
title_short Simultaneous Determination of Glibenclamide and Silymarin Released from Chitosan Microparticles by HPLC-ESI-MS Technique: Method Development and Validation
title_sort simultaneous determination of glibenclamide and silymarin released from chitosan microparticles by hplc-esi-ms technique: method development and validation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9611085/
https://www.ncbi.nlm.nih.gov/pubmed/36297600
http://dx.doi.org/10.3390/pharmaceutics14102164
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