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Development of a Well-Characterized Cynomolgus Macaque Model of Sudan Virus Disease for Support of Product Development
The primary objective of this study was to characterize the disease course in cynomolgus macaques exposed to Sudan virus (SUDV), to determine if infection in this species is an appropriate model for the evaluation of filovirus countermeasures under the FDA Animal Rule. Sudan virus causes Sudan virus...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9611481/ https://www.ncbi.nlm.nih.gov/pubmed/36298588 http://dx.doi.org/10.3390/vaccines10101723 |
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author | Alfson, Kendra J. Goez-Gazi, Yenny Gazi, Michal Chou, Ying-Liang Niemuth, Nancy A. Mattix, Marc E. Staples, Hilary Klaffke, Benjamin Rodriguez, Gloria F. Escareno, Priscilla Bartley, Carmen Ticer, Anysha Clemmons, Elizabeth A. Dutton III, John W. Griffiths, Anthony Meister, Gabe T. Sanford, Daniel C. Cirimotich, Chris M. Carrion, Ricardo |
author_facet | Alfson, Kendra J. Goez-Gazi, Yenny Gazi, Michal Chou, Ying-Liang Niemuth, Nancy A. Mattix, Marc E. Staples, Hilary Klaffke, Benjamin Rodriguez, Gloria F. Escareno, Priscilla Bartley, Carmen Ticer, Anysha Clemmons, Elizabeth A. Dutton III, John W. Griffiths, Anthony Meister, Gabe T. Sanford, Daniel C. Cirimotich, Chris M. Carrion, Ricardo |
author_sort | Alfson, Kendra J. |
collection | PubMed |
description | The primary objective of this study was to characterize the disease course in cynomolgus macaques exposed to Sudan virus (SUDV), to determine if infection in this species is an appropriate model for the evaluation of filovirus countermeasures under the FDA Animal Rule. Sudan virus causes Sudan virus disease (SVD), with an average case fatality rate of approximately 50%, and while research is ongoing, presently there are no approved SUDV vaccines or therapies. Well characterized animal models are crucial for further developing and evaluating countermeasures for SUDV. Twenty (20) cynomolgus macaques were exposed intramuscularly to either SUDV or sterile phosphate-buffered saline; 10 SUDV-exposed animals were euthanized on schedule to characterize pathology at defined durations post-exposure and 8 SUDV-exposed animals were not part of the scheduled euthanasia cohort. Survival was assessed, along with clinical observations, body weights, body temperatures, hematology, clinical chemistry, coagulation, viral load (serum and tissues), macroscopic observations, and histopathology. There were statistically significant differences between SUDV-exposed animals and mock-exposed animals for 26 parameters, including telemetry body temperature, clinical chemistry parameters, hematology parameters, activated partial thromboplastin time, serum viremia, and biomarkers that characterize the disease course of SUDV in cynomolgus macaques. |
format | Online Article Text |
id | pubmed-9611481 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96114812022-10-28 Development of a Well-Characterized Cynomolgus Macaque Model of Sudan Virus Disease for Support of Product Development Alfson, Kendra J. Goez-Gazi, Yenny Gazi, Michal Chou, Ying-Liang Niemuth, Nancy A. Mattix, Marc E. Staples, Hilary Klaffke, Benjamin Rodriguez, Gloria F. Escareno, Priscilla Bartley, Carmen Ticer, Anysha Clemmons, Elizabeth A. Dutton III, John W. Griffiths, Anthony Meister, Gabe T. Sanford, Daniel C. Cirimotich, Chris M. Carrion, Ricardo Vaccines (Basel) Article The primary objective of this study was to characterize the disease course in cynomolgus macaques exposed to Sudan virus (SUDV), to determine if infection in this species is an appropriate model for the evaluation of filovirus countermeasures under the FDA Animal Rule. Sudan virus causes Sudan virus disease (SVD), with an average case fatality rate of approximately 50%, and while research is ongoing, presently there are no approved SUDV vaccines or therapies. Well characterized animal models are crucial for further developing and evaluating countermeasures for SUDV. Twenty (20) cynomolgus macaques were exposed intramuscularly to either SUDV or sterile phosphate-buffered saline; 10 SUDV-exposed animals were euthanized on schedule to characterize pathology at defined durations post-exposure and 8 SUDV-exposed animals were not part of the scheduled euthanasia cohort. Survival was assessed, along with clinical observations, body weights, body temperatures, hematology, clinical chemistry, coagulation, viral load (serum and tissues), macroscopic observations, and histopathology. There were statistically significant differences between SUDV-exposed animals and mock-exposed animals for 26 parameters, including telemetry body temperature, clinical chemistry parameters, hematology parameters, activated partial thromboplastin time, serum viremia, and biomarkers that characterize the disease course of SUDV in cynomolgus macaques. MDPI 2022-10-15 /pmc/articles/PMC9611481/ /pubmed/36298588 http://dx.doi.org/10.3390/vaccines10101723 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Alfson, Kendra J. Goez-Gazi, Yenny Gazi, Michal Chou, Ying-Liang Niemuth, Nancy A. Mattix, Marc E. Staples, Hilary Klaffke, Benjamin Rodriguez, Gloria F. Escareno, Priscilla Bartley, Carmen Ticer, Anysha Clemmons, Elizabeth A. Dutton III, John W. Griffiths, Anthony Meister, Gabe T. Sanford, Daniel C. Cirimotich, Chris M. Carrion, Ricardo Development of a Well-Characterized Cynomolgus Macaque Model of Sudan Virus Disease for Support of Product Development |
title | Development of a Well-Characterized Cynomolgus Macaque Model of Sudan Virus Disease for Support of Product Development |
title_full | Development of a Well-Characterized Cynomolgus Macaque Model of Sudan Virus Disease for Support of Product Development |
title_fullStr | Development of a Well-Characterized Cynomolgus Macaque Model of Sudan Virus Disease for Support of Product Development |
title_full_unstemmed | Development of a Well-Characterized Cynomolgus Macaque Model of Sudan Virus Disease for Support of Product Development |
title_short | Development of a Well-Characterized Cynomolgus Macaque Model of Sudan Virus Disease for Support of Product Development |
title_sort | development of a well-characterized cynomolgus macaque model of sudan virus disease for support of product development |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9611481/ https://www.ncbi.nlm.nih.gov/pubmed/36298588 http://dx.doi.org/10.3390/vaccines10101723 |
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