Cargando…
Immunogenicity, Safety, and Anti-Viral Efficacy of a Subunit SARS-CoV-2 Vaccine Candidate in Captive Black-Footed Ferrets (Mustela nigripes) and Their Susceptibility to Viral Challenge
A preliminary vaccination trial against the emergent pathogen, SARS-CoV-2, was completed in captive black-footed ferrets (Mustela nigripes; BFF) to assess safety, immunogenicity, and anti-viral efficacy. Vaccination and boosting of 15 BFF with purified SARS-CoV-2 S1 subunit protein produced a nearly...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9612080/ https://www.ncbi.nlm.nih.gov/pubmed/36298743 http://dx.doi.org/10.3390/v14102188 |
_version_ | 1784819689409478656 |
---|---|
author | Leon, Ariel E. Garelle, Della Hartwig, Airn Falendysz, Elizabeth A. Ip, Hon S. Lankton, Julia S. Tretten, Tyler N. Spraker, Terry R. Bowen, Richard Rocke, Tonie E. |
author_facet | Leon, Ariel E. Garelle, Della Hartwig, Airn Falendysz, Elizabeth A. Ip, Hon S. Lankton, Julia S. Tretten, Tyler N. Spraker, Terry R. Bowen, Richard Rocke, Tonie E. |
author_sort | Leon, Ariel E. |
collection | PubMed |
description | A preliminary vaccination trial against the emergent pathogen, SARS-CoV-2, was completed in captive black-footed ferrets (Mustela nigripes; BFF) to assess safety, immunogenicity, and anti-viral efficacy. Vaccination and boosting of 15 BFF with purified SARS-CoV-2 S1 subunit protein produced a nearly 150-fold increase in mean antibody titers compared to pre-vaccination titers. Serum antibody responses were highest in young animals, but in all vaccinees, antibody response declined rapidly. Anti-viral activity from vaccinated and unvaccinated BFF was determined in vitro, as well as in vivo with a passive serum transfer study in mice. Transgenic mice that received BFF serum transfers and were subsequently challenged with SARS-CoV-2 had lung viral loads that negatively correlated (p < 0.05) with the BFF serum titer received. Lastly, an experimental challenge study in a small group of BFF was completed to test susceptibility to SARS-CoV-2. Despite viral replication and shedding in the upper respiratory tract for up to 7 days post-challenge, no clinical disease was observed in either vaccinated or naive animals. The lack of morbidity or mortality observed indicates SARS-CoV-2 is unlikely to affect wild BFF populations, but infected captive animals pose a potential risk, albeit low, for humans and other animals. |
format | Online Article Text |
id | pubmed-9612080 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96120802022-10-28 Immunogenicity, Safety, and Anti-Viral Efficacy of a Subunit SARS-CoV-2 Vaccine Candidate in Captive Black-Footed Ferrets (Mustela nigripes) and Their Susceptibility to Viral Challenge Leon, Ariel E. Garelle, Della Hartwig, Airn Falendysz, Elizabeth A. Ip, Hon S. Lankton, Julia S. Tretten, Tyler N. Spraker, Terry R. Bowen, Richard Rocke, Tonie E. Viruses Article A preliminary vaccination trial against the emergent pathogen, SARS-CoV-2, was completed in captive black-footed ferrets (Mustela nigripes; BFF) to assess safety, immunogenicity, and anti-viral efficacy. Vaccination and boosting of 15 BFF with purified SARS-CoV-2 S1 subunit protein produced a nearly 150-fold increase in mean antibody titers compared to pre-vaccination titers. Serum antibody responses were highest in young animals, but in all vaccinees, antibody response declined rapidly. Anti-viral activity from vaccinated and unvaccinated BFF was determined in vitro, as well as in vivo with a passive serum transfer study in mice. Transgenic mice that received BFF serum transfers and were subsequently challenged with SARS-CoV-2 had lung viral loads that negatively correlated (p < 0.05) with the BFF serum titer received. Lastly, an experimental challenge study in a small group of BFF was completed to test susceptibility to SARS-CoV-2. Despite viral replication and shedding in the upper respiratory tract for up to 7 days post-challenge, no clinical disease was observed in either vaccinated or naive animals. The lack of morbidity or mortality observed indicates SARS-CoV-2 is unlikely to affect wild BFF populations, but infected captive animals pose a potential risk, albeit low, for humans and other animals. MDPI 2022-10-04 /pmc/articles/PMC9612080/ /pubmed/36298743 http://dx.doi.org/10.3390/v14102188 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Leon, Ariel E. Garelle, Della Hartwig, Airn Falendysz, Elizabeth A. Ip, Hon S. Lankton, Julia S. Tretten, Tyler N. Spraker, Terry R. Bowen, Richard Rocke, Tonie E. Immunogenicity, Safety, and Anti-Viral Efficacy of a Subunit SARS-CoV-2 Vaccine Candidate in Captive Black-Footed Ferrets (Mustela nigripes) and Their Susceptibility to Viral Challenge |
title | Immunogenicity, Safety, and Anti-Viral Efficacy of a Subunit SARS-CoV-2 Vaccine Candidate in Captive Black-Footed Ferrets (Mustela nigripes) and Their Susceptibility to Viral Challenge |
title_full | Immunogenicity, Safety, and Anti-Viral Efficacy of a Subunit SARS-CoV-2 Vaccine Candidate in Captive Black-Footed Ferrets (Mustela nigripes) and Their Susceptibility to Viral Challenge |
title_fullStr | Immunogenicity, Safety, and Anti-Viral Efficacy of a Subunit SARS-CoV-2 Vaccine Candidate in Captive Black-Footed Ferrets (Mustela nigripes) and Their Susceptibility to Viral Challenge |
title_full_unstemmed | Immunogenicity, Safety, and Anti-Viral Efficacy of a Subunit SARS-CoV-2 Vaccine Candidate in Captive Black-Footed Ferrets (Mustela nigripes) and Their Susceptibility to Viral Challenge |
title_short | Immunogenicity, Safety, and Anti-Viral Efficacy of a Subunit SARS-CoV-2 Vaccine Candidate in Captive Black-Footed Ferrets (Mustela nigripes) and Their Susceptibility to Viral Challenge |
title_sort | immunogenicity, safety, and anti-viral efficacy of a subunit sars-cov-2 vaccine candidate in captive black-footed ferrets (mustela nigripes) and their susceptibility to viral challenge |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9612080/ https://www.ncbi.nlm.nih.gov/pubmed/36298743 http://dx.doi.org/10.3390/v14102188 |
work_keys_str_mv | AT leonariele immunogenicitysafetyandantiviralefficacyofasubunitsarscov2vaccinecandidateincaptiveblackfootedferretsmustelanigripesandtheirsusceptibilitytoviralchallenge AT garelledella immunogenicitysafetyandantiviralefficacyofasubunitsarscov2vaccinecandidateincaptiveblackfootedferretsmustelanigripesandtheirsusceptibilitytoviralchallenge AT hartwigairn immunogenicitysafetyandantiviralefficacyofasubunitsarscov2vaccinecandidateincaptiveblackfootedferretsmustelanigripesandtheirsusceptibilitytoviralchallenge AT falendyszelizabetha immunogenicitysafetyandantiviralefficacyofasubunitsarscov2vaccinecandidateincaptiveblackfootedferretsmustelanigripesandtheirsusceptibilitytoviralchallenge AT iphons immunogenicitysafetyandantiviralefficacyofasubunitsarscov2vaccinecandidateincaptiveblackfootedferretsmustelanigripesandtheirsusceptibilitytoviralchallenge AT lanktonjulias immunogenicitysafetyandantiviralefficacyofasubunitsarscov2vaccinecandidateincaptiveblackfootedferretsmustelanigripesandtheirsusceptibilitytoviralchallenge AT trettentylern immunogenicitysafetyandantiviralefficacyofasubunitsarscov2vaccinecandidateincaptiveblackfootedferretsmustelanigripesandtheirsusceptibilitytoviralchallenge AT sprakerterryr immunogenicitysafetyandantiviralefficacyofasubunitsarscov2vaccinecandidateincaptiveblackfootedferretsmustelanigripesandtheirsusceptibilitytoviralchallenge AT bowenrichard immunogenicitysafetyandantiviralefficacyofasubunitsarscov2vaccinecandidateincaptiveblackfootedferretsmustelanigripesandtheirsusceptibilitytoviralchallenge AT rocketoniee immunogenicitysafetyandantiviralefficacyofasubunitsarscov2vaccinecandidateincaptiveblackfootedferretsmustelanigripesandtheirsusceptibilitytoviralchallenge |