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Characterization of multiple interactions between the envelope E protein of SARS-CoV-2 and human BRD4
The SARS-CoV-2 envelope (E) protein hijacks human BRD4 (bromodomain and extra-terminal domain protein 4). Here, we describe a protocol to characterize the interaction of the acetylated E protein with BRD4 in vivo. We detail steps to use NMR spectroscopy to map the binding interface and include steps...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9613806/ https://www.ncbi.nlm.nih.gov/pubmed/36595918 http://dx.doi.org/10.1016/j.xpro.2022.101853 |