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Characterization of multiple interactions between the envelope E protein of SARS-CoV-2 and human BRD4

The SARS-CoV-2 envelope (E) protein hijacks human BRD4 (bromodomain and extra-terminal domain protein 4). Here, we describe a protocol to characterize the interaction of the acetylated E protein with BRD4 in vivo. We detail steps to use NMR spectroscopy to map the binding interface and include steps...

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Detalles Bibliográficos
Autores principales:	Zandian, Mohamad, Jang, Suk Min, Lachance, Catherine, Acharya, Arpan, Byrareddy, Siddappa N., Côté, Jacques, Kutateladze, Tatiana G.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9613806/ https://www.ncbi.nlm.nih.gov/pubmed/36595918 http://dx.doi.org/10.1016/j.xpro.2022.101853

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