The efficient generation of knockout microglia cells using a dual-sgRNA strategy by CRISPR/Cas9

Gene deletion in microglia has become an important and exciting approach for studying neuroinflammation, especially after the development of the CRISPR/Cas9 system for genome editing during the last decade. In this study, we described a protocol for the highly efficient generation of knockout microg...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Mengfei, Yi, Fang, Wu, Junjiao, Tang, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Molecular Neuroscience
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9614382/
https://www.ncbi.nlm.nih.gov/pubmed/36311032
http://dx.doi.org/10.3389/fnmol.2022.1008827

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9614382/
https://www.ncbi.nlm.nih.gov/pubmed/36311032
http://dx.doi.org/10.3389/fnmol.2022.1008827

Ejemplares similares