Cargando…
The efficient generation of knockout microglia cells using a dual-sgRNA strategy by CRISPR/Cas9
Gene deletion in microglia has become an important and exciting approach for studying neuroinflammation, especially after the development of the CRISPR/Cas9 system for genome editing during the last decade. In this study, we described a protocol for the highly efficient generation of knockout microg...
| Autores principales: | , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Frontiers Media S.A.
2022
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9614382/ https://www.ncbi.nlm.nih.gov/pubmed/36311032 http://dx.doi.org/10.3389/fnmol.2022.1008827 |