High-Throughput Glycomic Methods

[Image: see text] Glycomics aims to identify the structure and function of the glycome, the complete set of oligosaccharides (glycans), produced in a given cell or organism, as well as to identify genes and other factors that govern glycosylation. This challenging endeavor requires highly robust, se...

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Detalles Bibliográficos
Autores principales: Trbojević-Akmačić, Irena, Lageveen-Kammeijer, Guinevere S. M., Heijs, Bram, Petrović, Tea, Deriš, Helena, Wuhrer, Manfred, Lauc, Gordan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9614987/
https://www.ncbi.nlm.nih.gov/pubmed/35797639
http://dx.doi.org/10.1021/acs.chemrev.1c01031
Descripción
Sumario:[Image: see text] Glycomics aims to identify the structure and function of the glycome, the complete set of oligosaccharides (glycans), produced in a given cell or organism, as well as to identify genes and other factors that govern glycosylation. This challenging endeavor requires highly robust, sensitive, and potentially automatable analytical technologies for the analysis of hundreds or thousands of glycomes in a timely manner (termed high-throughput glycomics). This review provides a historic overview as well as highlights recent developments and challenges of glycomic profiling by the most prominent high-throughput glycomic approaches, with N-glycosylation analysis as the focal point. It describes the current state-of-the-art regarding levels of characterization and most widely used technologies, selected applications of high-throughput glycomics in deciphering glycosylation process in healthy and disease states, as well as future perspectives.