Efficient RNA and RNA-protein co-detection in 3D colonoids by whole-mount staining

Here, we describe a protocol to visualize RNA oligos and proteins independently or together using a combination of fluorescence in situ hybridization (FISH) and immunofluorescence in human colonoids, expanding on previously published research. Whole-mount staining is used to preserve the colonoid st...

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Detalles Bibliográficos
Autores principales: Atanga, Roger, Parra, Amalia S., In, Julie G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9615320/
https://www.ncbi.nlm.nih.gov/pubmed/36313534
http://dx.doi.org/10.1016/j.xpro.2022.101775

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9615320/
https://www.ncbi.nlm.nih.gov/pubmed/36313534
http://dx.doi.org/10.1016/j.xpro.2022.101775

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