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Efficient RNA and RNA-protein co-detection in 3D colonoids by whole-mount staining
Here, we describe a protocol to visualize RNA oligos and proteins independently or together using a combination of fluorescence in situ hybridization (FISH) and immunofluorescence in human colonoids, expanding on previously published research. Whole-mount staining is used to preserve the colonoid st...
Autores principales: | Atanga, Roger, Parra, Amalia S., In, Julie G. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9615320/ https://www.ncbi.nlm.nih.gov/pubmed/36313534 http://dx.doi.org/10.1016/j.xpro.2022.101775 |
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