Cargando…

A satellite DNA array barcodes chromosome 7 and regulates totipotency via ZFP819

Mammalian genomes are a battleground for genetic conflict between repetitive elements and KRAB-zinc finger proteins (KZFPs). We asked whether KZFPs can regulate cell fate by using ZFP819, which targets a satellite DNA array, ZP3AR. ZP3AR coats megabase regions of chromosome 7 encompassing genes enco...

Descripción completa

Detalles Bibliográficos
Autores principales: Fernandes, Liane P., Enriquez-Gasca, Rocio, Gould, Poppy A., Holt, James H., Conde, Lucia, Ecco, Gabriela, Herrero, Javier, Gifford, Robert, Trono, Didier, Kassiotis, George, Rowe, Helen M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9616502/
https://www.ncbi.nlm.nih.gov/pubmed/36306355
http://dx.doi.org/10.1126/sciadv.abp8085
_version_ 1784820655628222464
author Fernandes, Liane P.
Enriquez-Gasca, Rocio
Gould, Poppy A.
Holt, James H.
Conde, Lucia
Ecco, Gabriela
Herrero, Javier
Gifford, Robert
Trono, Didier
Kassiotis, George
Rowe, Helen M.
author_facet Fernandes, Liane P.
Enriquez-Gasca, Rocio
Gould, Poppy A.
Holt, James H.
Conde, Lucia
Ecco, Gabriela
Herrero, Javier
Gifford, Robert
Trono, Didier
Kassiotis, George
Rowe, Helen M.
author_sort Fernandes, Liane P.
collection PubMed
description Mammalian genomes are a battleground for genetic conflict between repetitive elements and KRAB-zinc finger proteins (KZFPs). We asked whether KZFPs can regulate cell fate by using ZFP819, which targets a satellite DNA array, ZP3AR. ZP3AR coats megabase regions of chromosome 7 encompassing genes encoding ZSCAN4, a master transcription factor of totipotency. Depleting ZFP819 in mouse embryonic stem cells (mESCs) causes them to transition to a 2-cell (2C)–like state, whereby the ZP3AR array switches from a poised to an active enhancer state. This is accompanied by a global erosion of heterochromatin roadblocks, which we link to decreased SETDB1 stability. These events result in transcription of active LINE-1 elements and impaired differentiation. In summary, ZFP819 and TRIM28 partner up to close chromatin across Zscan4, to promote exit from totipotency. We propose that satellite DNAs may control developmental fate transitions by barcoding and switching off master transcription factor genes.
format Online
Article
Text
id pubmed-9616502
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher American Association for the Advancement of Science
record_format MEDLINE/PubMed
spelling pubmed-96165022022-11-04 A satellite DNA array barcodes chromosome 7 and regulates totipotency via ZFP819 Fernandes, Liane P. Enriquez-Gasca, Rocio Gould, Poppy A. Holt, James H. Conde, Lucia Ecco, Gabriela Herrero, Javier Gifford, Robert Trono, Didier Kassiotis, George Rowe, Helen M. Sci Adv Biomedicine and Life Sciences Mammalian genomes are a battleground for genetic conflict between repetitive elements and KRAB-zinc finger proteins (KZFPs). We asked whether KZFPs can regulate cell fate by using ZFP819, which targets a satellite DNA array, ZP3AR. ZP3AR coats megabase regions of chromosome 7 encompassing genes encoding ZSCAN4, a master transcription factor of totipotency. Depleting ZFP819 in mouse embryonic stem cells (mESCs) causes them to transition to a 2-cell (2C)–like state, whereby the ZP3AR array switches from a poised to an active enhancer state. This is accompanied by a global erosion of heterochromatin roadblocks, which we link to decreased SETDB1 stability. These events result in transcription of active LINE-1 elements and impaired differentiation. In summary, ZFP819 and TRIM28 partner up to close chromatin across Zscan4, to promote exit from totipotency. We propose that satellite DNAs may control developmental fate transitions by barcoding and switching off master transcription factor genes. American Association for the Advancement of Science 2022-10-28 /pmc/articles/PMC9616502/ /pubmed/36306355 http://dx.doi.org/10.1126/sciadv.abp8085 Text en Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Biomedicine and Life Sciences
Fernandes, Liane P.
Enriquez-Gasca, Rocio
Gould, Poppy A.
Holt, James H.
Conde, Lucia
Ecco, Gabriela
Herrero, Javier
Gifford, Robert
Trono, Didier
Kassiotis, George
Rowe, Helen M.
A satellite DNA array barcodes chromosome 7 and regulates totipotency via ZFP819
title A satellite DNA array barcodes chromosome 7 and regulates totipotency via ZFP819
title_full A satellite DNA array barcodes chromosome 7 and regulates totipotency via ZFP819
title_fullStr A satellite DNA array barcodes chromosome 7 and regulates totipotency via ZFP819
title_full_unstemmed A satellite DNA array barcodes chromosome 7 and regulates totipotency via ZFP819
title_short A satellite DNA array barcodes chromosome 7 and regulates totipotency via ZFP819
title_sort satellite dna array barcodes chromosome 7 and regulates totipotency via zfp819
topic Biomedicine and Life Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9616502/
https://www.ncbi.nlm.nih.gov/pubmed/36306355
http://dx.doi.org/10.1126/sciadv.abp8085
work_keys_str_mv AT fernandeslianep asatellitednaarraybarcodeschromosome7andregulatestotipotencyviazfp819
AT enriquezgascarocio asatellitednaarraybarcodeschromosome7andregulatestotipotencyviazfp819
AT gouldpoppya asatellitednaarraybarcodeschromosome7andregulatestotipotencyviazfp819
AT holtjamesh asatellitednaarraybarcodeschromosome7andregulatestotipotencyviazfp819
AT condelucia asatellitednaarraybarcodeschromosome7andregulatestotipotencyviazfp819
AT eccogabriela asatellitednaarraybarcodeschromosome7andregulatestotipotencyviazfp819
AT herrerojavier asatellitednaarraybarcodeschromosome7andregulatestotipotencyviazfp819
AT giffordrobert asatellitednaarraybarcodeschromosome7andregulatestotipotencyviazfp819
AT tronodidier asatellitednaarraybarcodeschromosome7andregulatestotipotencyviazfp819
AT kassiotisgeorge asatellitednaarraybarcodeschromosome7andregulatestotipotencyviazfp819
AT rowehelenm asatellitednaarraybarcodeschromosome7andregulatestotipotencyviazfp819
AT fernandeslianep satellitednaarraybarcodeschromosome7andregulatestotipotencyviazfp819
AT enriquezgascarocio satellitednaarraybarcodeschromosome7andregulatestotipotencyviazfp819
AT gouldpoppya satellitednaarraybarcodeschromosome7andregulatestotipotencyviazfp819
AT holtjamesh satellitednaarraybarcodeschromosome7andregulatestotipotencyviazfp819
AT condelucia satellitednaarraybarcodeschromosome7andregulatestotipotencyviazfp819
AT eccogabriela satellitednaarraybarcodeschromosome7andregulatestotipotencyviazfp819
AT herrerojavier satellitednaarraybarcodeschromosome7andregulatestotipotencyviazfp819
AT giffordrobert satellitednaarraybarcodeschromosome7andregulatestotipotencyviazfp819
AT tronodidier satellitednaarraybarcodeschromosome7andregulatestotipotencyviazfp819
AT kassiotisgeorge satellitednaarraybarcodeschromosome7andregulatestotipotencyviazfp819
AT rowehelenm satellitednaarraybarcodeschromosome7andregulatestotipotencyviazfp819