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Quantifying transport dynamics with three-dimensional single-particle tracking in adherent cells
Intracellular transport plays an important role in maintaining the physiological functions of cells. Here, we describe a protocol for 3D single-particle tracking within living cells. We detail the use of a two-focal imaging system and the analytical steps for quantifying 3D transport dynamics. This...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9617199/ https://www.ncbi.nlm.nih.gov/pubmed/36317175 http://dx.doi.org/10.1016/j.xpro.2022.101790 |