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Quantifying transport dynamics with three-dimensional single-particle tracking in adherent cells

Intracellular transport plays an important role in maintaining the physiological functions of cells. Here, we describe a protocol for 3D single-particle tracking within living cells. We detail the use of a two-focal imaging system and the analytical steps for quantifying 3D transport dynamics. This...

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Detalles Bibliográficos
Autores principales: Jiang, Chao, Dou, Shuo-Xing, Wang, Peng-Ye, Li, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9617199/
https://www.ncbi.nlm.nih.gov/pubmed/36317175
http://dx.doi.org/10.1016/j.xpro.2022.101790