Quantifying transport dynamics with three-dimensional single-particle tracking in adherent cells

Intracellular transport plays an important role in maintaining the physiological functions of cells. Here, we describe a protocol for 3D single-particle tracking within living cells. We detail the use of a two-focal imaging system and the analytical steps for quantifying 3D transport dynamics. This...

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Detalles Bibliográficos
Autores principales: Jiang, Chao, Dou, Shuo-Xing, Wang, Peng-Ye, Li, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9617199/
https://www.ncbi.nlm.nih.gov/pubmed/36317175
http://dx.doi.org/10.1016/j.xpro.2022.101790
Descripción
Sumario:Intracellular transport plays an important role in maintaining the physiological functions of cells. Here, we describe a protocol for 3D single-particle tracking within living cells. We detail the use of a two-focal imaging system and the analytical steps for quantifying 3D transport dynamics. This protocol can be used to characterize the intracellular diffusion and trafficking of macromolecules, nanoparticles, and endocytic vesicles in adherent cells. For complete details on the use and execution of this protocol, please refer to Jiang et al. (2022).

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