Quantifying transport dynamics with three-dimensional single-particle tracking in adherent cells

Intracellular transport plays an important role in maintaining the physiological functions of cells. Here, we describe a protocol for 3D single-particle tracking within living cells. We detail the use of a two-focal imaging system and the analytical steps for quantifying 3D transport dynamics. This...

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Detalles Bibliográficos
Autores principales: Jiang, Chao, Dou, Shuo-Xing, Wang, Peng-Ye, Li, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9617199/
https://www.ncbi.nlm.nih.gov/pubmed/36317175
http://dx.doi.org/10.1016/j.xpro.2022.101790
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author Jiang, Chao
Dou, Shuo-Xing
Wang, Peng-Ye
Li, Hui
author_facet Jiang, Chao
Dou, Shuo-Xing
Wang, Peng-Ye
Li, Hui
author_sort Jiang, Chao
collection PubMed
description Intracellular transport plays an important role in maintaining the physiological functions of cells. Here, we describe a protocol for 3D single-particle tracking within living cells. We detail the use of a two-focal imaging system and the analytical steps for quantifying 3D transport dynamics. This protocol can be used to characterize the intracellular diffusion and trafficking of macromolecules, nanoparticles, and endocytic vesicles in adherent cells. For complete details on the use and execution of this protocol, please refer to Jiang et al. (2022).
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spelling pubmed-96171992022-10-30 Quantifying transport dynamics with three-dimensional single-particle tracking in adherent cells Jiang, Chao Dou, Shuo-Xing Wang, Peng-Ye Li, Hui STAR Protoc Protocol Intracellular transport plays an important role in maintaining the physiological functions of cells. Here, we describe a protocol for 3D single-particle tracking within living cells. We detail the use of a two-focal imaging system and the analytical steps for quantifying 3D transport dynamics. This protocol can be used to characterize the intracellular diffusion and trafficking of macromolecules, nanoparticles, and endocytic vesicles in adherent cells. For complete details on the use and execution of this protocol, please refer to Jiang et al. (2022). Elsevier 2022-10-26 /pmc/articles/PMC9617199/ /pubmed/36317175 http://dx.doi.org/10.1016/j.xpro.2022.101790 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Jiang, Chao
Dou, Shuo-Xing
Wang, Peng-Ye
Li, Hui
Quantifying transport dynamics with three-dimensional single-particle tracking in adherent cells
title Quantifying transport dynamics with three-dimensional single-particle tracking in adherent cells
title_full Quantifying transport dynamics with three-dimensional single-particle tracking in adherent cells
title_fullStr Quantifying transport dynamics with three-dimensional single-particle tracking in adherent cells
title_full_unstemmed Quantifying transport dynamics with three-dimensional single-particle tracking in adherent cells
title_short Quantifying transport dynamics with three-dimensional single-particle tracking in adherent cells
title_sort quantifying transport dynamics with three-dimensional single-particle tracking in adherent cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9617199/
https://www.ncbi.nlm.nih.gov/pubmed/36317175
http://dx.doi.org/10.1016/j.xpro.2022.101790
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AT wangpengye quantifyingtransportdynamicswiththreedimensionalsingleparticletrackinginadherentcells
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