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A protocol to induce expandable limb-bud mesenchymal cells from human pluripotent stem cells

Here, we present a protocol for the selective differentiation of human pluripotent stem cells mimicking human developmental processes into expandable PRRX1(+) limb-bud mesenchymal (ExpLBM) cells. This approach enables expansion through serial passage while maintaining capacity for chondrogenic diffe...

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Detalles Bibliográficos
Autores principales: Takao, Tomoka, Yamada, Daisuke, Takarada, Takeshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9617203/
https://www.ncbi.nlm.nih.gov/pubmed/36317170
http://dx.doi.org/10.1016/j.xpro.2022.101786