A protocol to induce expandable limb-bud mesenchymal cells from human pluripotent stem cells

Here, we present a protocol for the selective differentiation of human pluripotent stem cells mimicking human developmental processes into expandable PRRX1(+) limb-bud mesenchymal (ExpLBM) cells. This approach enables expansion through serial passage while maintaining capacity for chondrogenic diffe...

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Detalles Bibliográficos
Autores principales: Takao, Tomoka, Yamada, Daisuke, Takarada, Takeshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9617203/
https://www.ncbi.nlm.nih.gov/pubmed/36317170
http://dx.doi.org/10.1016/j.xpro.2022.101786
Descripción
Sumario:Here, we present a protocol for the selective differentiation of human pluripotent stem cells mimicking human developmental processes into expandable PRRX1(+) limb-bud mesenchymal (ExpLBM) cells. This approach enables expansion through serial passage while maintaining capacity for chondrogenic differentiation. For complete details on the use and execution of this protocol, please refer to Yamada et al. (2021, 2022).

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