A protocol to induce expandable limb-bud mesenchymal cells from human pluripotent stem cells

Here, we present a protocol for the selective differentiation of human pluripotent stem cells mimicking human developmental processes into expandable PRRX1(+) limb-bud mesenchymal (ExpLBM) cells. This approach enables expansion through serial passage while maintaining capacity for chondrogenic diffe...

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Detalles Bibliográficos
Autores principales: Takao, Tomoka, Yamada, Daisuke, Takarada, Takeshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9617203/
https://www.ncbi.nlm.nih.gov/pubmed/36317170
http://dx.doi.org/10.1016/j.xpro.2022.101786
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author Takao, Tomoka
Yamada, Daisuke
Takarada, Takeshi
author_facet Takao, Tomoka
Yamada, Daisuke
Takarada, Takeshi
author_sort Takao, Tomoka
collection PubMed
description Here, we present a protocol for the selective differentiation of human pluripotent stem cells mimicking human developmental processes into expandable PRRX1(+) limb-bud mesenchymal (ExpLBM) cells. This approach enables expansion through serial passage while maintaining capacity for chondrogenic differentiation. For complete details on the use and execution of this protocol, please refer to Yamada et al. (2021, 2022).
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spelling pubmed-96172032022-10-30 A protocol to induce expandable limb-bud mesenchymal cells from human pluripotent stem cells Takao, Tomoka Yamada, Daisuke Takarada, Takeshi STAR Protoc Protocol Here, we present a protocol for the selective differentiation of human pluripotent stem cells mimicking human developmental processes into expandable PRRX1(+) limb-bud mesenchymal (ExpLBM) cells. This approach enables expansion through serial passage while maintaining capacity for chondrogenic differentiation. For complete details on the use and execution of this protocol, please refer to Yamada et al. (2021, 2022). Elsevier 2022-10-26 /pmc/articles/PMC9617203/ /pubmed/36317170 http://dx.doi.org/10.1016/j.xpro.2022.101786 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Takao, Tomoka
Yamada, Daisuke
Takarada, Takeshi
A protocol to induce expandable limb-bud mesenchymal cells from human pluripotent stem cells
title A protocol to induce expandable limb-bud mesenchymal cells from human pluripotent stem cells
title_full A protocol to induce expandable limb-bud mesenchymal cells from human pluripotent stem cells
title_fullStr A protocol to induce expandable limb-bud mesenchymal cells from human pluripotent stem cells
title_full_unstemmed A protocol to induce expandable limb-bud mesenchymal cells from human pluripotent stem cells
title_short A protocol to induce expandable limb-bud mesenchymal cells from human pluripotent stem cells
title_sort protocol to induce expandable limb-bud mesenchymal cells from human pluripotent stem cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9617203/
https://www.ncbi.nlm.nih.gov/pubmed/36317170
http://dx.doi.org/10.1016/j.xpro.2022.101786
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