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Enhanced Acetate Tolerance and Recombinant Protein Accumulation in Escherichia coli by Transgenic Expression of a Heat Shock Protein from Carrot (Daucus carota L.)

BACKGROUND: In Escherichia coli (E. coli) culture, acetate accumulates as an undesirable by-product of aerobic fermentation on glucose and inhibits cell growth and recombinant protein production. OBJECTIVES: We examined whether the heterologous expression of a eukaryotic heat shock protein (Hsp) can...

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Detalles Bibliográficos
Autores principales: Kim, Minhye, Im, Eunju, Jin Ahn, Yeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Institute of Genetic Engineering and Biotechnology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9618011/
https://www.ncbi.nlm.nih.gov/pubmed/36381282
http://dx.doi.org/10.30498/ijb.2022.309657.3177