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Enhanced Acetate Tolerance and Recombinant Protein Accumulation in Escherichia coli by Transgenic Expression of a Heat Shock Protein from Carrot (Daucus carota L.)
BACKGROUND: In Escherichia coli (E. coli) culture, acetate accumulates as an undesirable by-product of aerobic fermentation on glucose and inhibits cell growth and recombinant protein production. OBJECTIVES: We examined whether the heterologous expression of a eukaryotic heat shock protein (Hsp) can...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Institute of Genetic Engineering and Biotechnology
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9618011/ https://www.ncbi.nlm.nih.gov/pubmed/36381282 http://dx.doi.org/10.30498/ijb.2022.309657.3177 |