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Vector redesign and in‐droplet cell‐growth improves enrichment and recovery in live Escherichia coli

Directed evolution (DE) is a widely used method for improving the function of biomolecules via multiple rounds of mutation and selection. Microfluidic droplets have emerged as an important means to screen the large libraries needed for DE, but this approach was so far partially limited by the need t...

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Detalles Bibliográficos
Autores principales: Eenink, Bernard D. G., Kaminski, Tomasz S., Bornberg‐Bauer, Erich, Jose, Joachim, Hollfelder, Florian, van Loo, Bert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9618318/
https://www.ncbi.nlm.nih.gov/pubmed/36099491
http://dx.doi.org/10.1111/1751-7915.14144