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Vector redesign and in‐droplet cell‐growth improves enrichment and recovery in live Escherichia coli
Directed evolution (DE) is a widely used method for improving the function of biomolecules via multiple rounds of mutation and selection. Microfluidic droplets have emerged as an important means to screen the large libraries needed for DE, but this approach was so far partially limited by the need t...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9618318/ https://www.ncbi.nlm.nih.gov/pubmed/36099491 http://dx.doi.org/10.1111/1751-7915.14144 |