Protocol for genome editing in wild allotetraploid rice Oryza alta

We present a protocol for Agrobacterium-mediated genetic transformation and genome editing in wild allotetraploid rice Oryza alta. We detail steps to induce embryogenic calli from mature seeds and co-cultivate with Agrobacterium after infection. We further describe how to select transformed cells th...

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Detalles Bibliográficos
Autores principales: Zhang, Jingkun, Zeng, Peng, Yu, Hong, Meng, Xiangbing, Li, Jiayang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9619720/
https://www.ncbi.nlm.nih.gov/pubmed/36325580
http://dx.doi.org/10.1016/j.xpro.2022.101789

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9619720/
https://www.ncbi.nlm.nih.gov/pubmed/36325580
http://dx.doi.org/10.1016/j.xpro.2022.101789

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